PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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+ o/ ^' z% x7 }! tPDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press
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主要内容如下：; L% ]& Z" W- j% U4 ~, l3 x
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Contents6 O4 A/ M$ @( m0 H: g3 w
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii
, C! k/ s0 _$ D2 QContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi, X$ H" {! _4 I+ Y
  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1
: l" \2 F( t2 A; nChristina Philippeos, Robin D. Hughes, Anil Dhawan, 5 q5 R4 R) H/ P$ i
and Ragai R. Mitry
' k7 X3 F- y% i+ E. M  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate ' t: a9 ?9 J9 x9 P* x
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15
2 P8 v# ?% H' FLing Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
. ~# Y' c1 _! u8 N; Z  3  Isolation, Cultivation, and Application of Human Alveolar & N- X, \  v# Y8 V0 X
Epithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31+ l8 K! N/ C: W3 R( l$ w" S: ~
Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer,
$ l0 `4 m5 V. w- N3 t3 r1 UHanno Huwer, and Claus-Michael Lehr
1 d  j3 M" i7 s. |5 L2 J  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43* v7 m9 j( n+ Y
Peyman Björklund and Per Hellman
; w( |( B* f5 c, y  5  Functional Analysis of Human Islets of Langerhans Maintained
  e1 U' S+ b2 R2 F$ @in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  557 @# K. Z2 y4 Q. p/ o& @
Shanta J. Persaud, Bo Liu, and Peter M. Jones
9 ]/ r0 C! Q. ~1 h  6  Conversion of Non-endocrine Human Pancreatic Cells
6 {. F1 m0 t( o$ g- [0 W, m: a) s* }to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73# n0 U5 v/ j. H4 F# x" j
Min Zhao and Guo Cai Huang* q1 N, ]) \  y- s* |8 j' h6 S8 T- H# F
  7  Evaluation of Cytochrome P450 Activities in Human $ G' M8 m% F# z+ `: H. J0 M) h
Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  87$ E& z0 c% w: G# x8 k
María José Gómez-Lechón, Agustín Lahoz, José V. Castell, ) T" L; D5 f8 _0 ]& f. b; ?
and María Teresa Donato
5 N% a9 p3 }/ n. @& A* q6 f  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99
( c' {- ^+ G2 YDaniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, " G9 V( k1 C$ l6 a4 G. I1 K4 z
Peter Büchler, Michael Schoenberg, Jörg Kleeff, 7 H+ O! H9 p' V$ N& l
Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, ; p, o! `' ?9 c
Jan Hengstler, and Andreas K. Nussler
6 K( ]: w# V7 k) D  9  Preclinical Testing of Virotherapeutics for Primary and Secondary . b3 Y1 a; v, n1 n6 }3 U( E! Z
Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121
7 k4 M! B3 A: F3 s1 U/ l3 FMartina Zimmermann, Timo Weiland, Michael Bitzer, 5 \- ^" m0 R$ M3 P- F! P( }
and Ulrich M. Lauer  Q' }: v0 F9 f  r5 p
10  Isolation and Functional Studies of Human Fetal Gastric
9 ]( h2 x7 j9 a' bEpithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
* J) q% y6 ~+ B5 W. UPierre Chailler, Jean-François Beaulieu, and Daniel Ménard# V" ]6 S7 \- ?. \% z7 h
11  Isolation, Characterization, and Culture of Normal Human + k6 J* P' P" L% r
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157
# J# `7 N* {0 ~5 Y5 c9 v9 T- ?6 EJean-François Beaulieu and Daniel Ménard! G% \9 Q! ^6 O( p! R
x Contents7 N$ I; c5 z- T7 k( M: y$ e1 k, q) K
12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells # ~( ]7 f- u* y1 J) ]) N
and Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1754 R8 L5 G6 Q$ f6 }  E
Claire C. Sharpe and Mark E.C. Dockrell
3 Z8 q2 s3 V, n7 p13  Glomerular Epithelial and Mesangial Cell Culture $ h6 ^$ {1 h: c. j/ A% j5 U' E" z
and Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187
! I+ P6 V3 N. \" u3 o, o) tHeather M. Wilson and Keith N. Stewart, W4 I7 l" V0 Z" s4 ?) x7 t+ W
14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203
; \+ L3 P( L) _: D& XKirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried
& |2 G, j- h* q, j15  Primary Culture of Human Adipocyte Precursor Cells: 0 q2 A9 r2 ~* G4 H! k. t
Expansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215
% l% y# k6 Z6 N- K4 Z: iThomas Skurk and Hans Hauner
+ h/ `- a4 e/ H16  Primary Culture of Ovarian Cells for Research on Cell Interactions
7 [8 q1 T& ~8 s; Hin the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227
1 l% F, I& D# WJerzy F. Galas
8 }( t( o; l1 F5 X) N17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251
3 Z# y1 L( v9 y7 H( C! FDiane Proudfoot and Catherine Shanahan+ m) w$ R) D. G" q% F* |
18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265
  }, c8 s( s9 ]Richard C.M. Siow
3 G8 a; C* c  h  O19  Human Peripheral Blood Mononuclear Cell Culture for Flow
& @% w9 E7 o- {Cytometric Analysis of Phosphorylated Mitogen-Activated
" O" j. N" I2 m( h& ZProtein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2752 D! d+ M- L) F$ e
Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou,
8 O3 S4 t3 d/ t# n- ]: @and Dimitrios P. Bogdanos
- B) n4 d0 n5 {, E) h+ S1 i- S20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287) T: f# t3 d: ~) i7 v1 E
Haibin Su, Maria Serena Longhi, Pengyun Wang,
$ B4 M& r0 R" }: f5 ?2 QDiego Vergani, and Yun Ma
% W+ q: [7 @; a9 S: V7 H( x21  Human Chondrocyte Cultures as Models of Cartilage-Specific " V5 r. Z* i( f& i* J; \4 F; H& b
Gene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301
% f" G3 X/ k% f: W4 LMiguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, 2 p, }7 A8 r8 l% Y& z. S
Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring* W/ l3 L6 K) E' ?4 k
22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337& v. h4 u8 L  d% Y* e
Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, 2 T1 o2 e/ ?0 e" L
and James A. Gallagher
1 P+ W8 W! ]/ r; v4 D$ X23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357
! |: c6 I+ U) g  sAnkita Agrawal, James A. Gallagher, and Alison Gartland& L6 J/ h* x" Z9 e6 f% e
24  Effects of Temperature Generated from the Holmium: YAG Laser 8 Z( X1 Q  @! i$ w$ `
on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377
! j  R/ D& |" W% _8 CMoustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, 0 G1 ]$ A* o( A3 u7 Y& E0 a
Ian D. McCarthy, and Al-Shymaa M. Hafez % n1 i/ X# d- T7 N* ~4 f2 J( E
25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  385& ?! A5 _$ m& d) Y2 v# H
Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia* {# o; E: ?% U( s
26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393" _1 E+ ]. w$ G) ^) u
Robert Thomas and Elizabeth Ratcliffe  ], L* a2 d$ L; @  ?7 `+ O
27  Culturing and Differentiating Human Mesenchymal Stem Cells 1 R4 B4 S* w- a3 I* v
for Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  407  z" J( e$ m! `0 R  E
William R. Otto and Catherine E. Sarraf
4 Y9 ^) f$ D9 x- K( m; `% [; lIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  4273 s7 x2 Z0 ^) p* z
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