PDF电子书：Ribosome display and related technologies （Springer MMB V805 2012）

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Ribosome display and related technologies : methods and protocols ; h8 r9 n, m9 G
edited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805)   
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8 j4 u( g/ N! G5 D% ?: s    Contents
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    Preface
/ ?+ w4 L  ]' G; T- v7 U# a    Contributors
. `& w6 I# r, [& i3 N$ `+ ]    PART I REVIEW
1 T+ ^: L& M- F# f! e    1 Ribosome Display: A Perspective! m" L% i; V. y
    PART II TRANSLATION EXTRACT PREPARATION2 ]; Q* n/ B3 {; a3 w
    2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts4 S7 s; D7 F" d
    PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS. O. M; q! S9 J/ i% A6 b$ [
    3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 45( G2 W  d3 a8 y2 y  @7 r
    4 Stabilized Ribosome Display for In Vitro Selection
3 W  q6 X1 G( l" y- x- q4 z    5 Eukaryotic Ribosome Display with In Situ DNA Recovery 75
8 D, K7 B, n- O# s) D- A, A    6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 87
) E9 q: }/ U  f7 t0 A4 g    7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101
7 h. c$ A& V" J2 K0 I  Y    8 cDNA Display: Rapid Stabilization of mRNA Display 113
) Q* n5 o' Z/ o( x- |0 e' @    PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS
9 s9 `8 w* W5 X% d    9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis! a8 m, R. X. G1 z9 D5 o$ e. @% B
    10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display
2 D/ s8 N9 m6 q6 d    11 Evolution of Protein Stability Using Ribosome Display0 F' y4 c3 b2 D/ O
    12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
; O, q! l, C9 _9 e    13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
/ Q4 M) |$ c2 D* M2 P, ~8 K, e- |    14 Peptide Screening Using PURE Ribosome Display/ o: Q2 i& w- T  _
    15 Rapid Selection of High-Affinity Binders Using Ribosome Display 261
! {# c$ Y2 U" Y    16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287
3 u$ ]- _. I- w    17 Identification of Candidate Vaccine Genes Using Ribosome Display 299
$ D8 t# o: X, O  d0 S    18 Ribosome Display for the Selection of Sac7d Scaffolds 315( k  D( {; C. ]1 f6 [6 F
    PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS" c! ?  l- P/ m2 O3 p
    19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335# H5 V5 k. T6 k0 f
    20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349
( v2 I0 t9 T6 C+ x& |    21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 3676 l0 w( |% k0 h
    PART VI CASE STUDIES
7 W" u9 o8 g0 B( T) i/ M, s3 Z    22 Optimization of CAT-354， a Therapeutic Antibody Directed Against Interleukin-13， Using Ribosome Display
' ]1 S2 ~1 [' C" b    23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 403; [1 B1 U5 |9 T; V! x; ?: K7 U2 B
    Index 423 : }+ J8 Y% D1 c1 W

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goast8

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immusic

有权限的限制，还是感谢！

ccbeyond

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