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本帖最后由 细胞海洋 于 2013-1-12 22:42 编辑
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" w( q) s8 N) {0 n% U* c$ \Ribosome display and related technologies : methods and protocols 8 a: d9 x8 M( j
edited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805) 7 q% d. S7 B+ E" G( V/ `
3 ~' u- l$ P+ K$ S Contents
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Preface+ D$ `7 u3 b2 d+ ~
Contributors
) T" v. R8 y" T4 e* B6 Q- U PART I REVIEW$ `3 s" f/ V; C7 y+ \
1 Ribosome Display: A Perspective
$ z" M! p5 C: ~" ]' O! d+ _$ B S PART II TRANSLATION EXTRACT PREPARATION
* v- U+ ^: U7 q 2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts6 I$ H9 J6 T% H7 o7 X
PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS0 v/ \9 b# ]# E/ v6 g& S I
3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 45
\7 q( @+ e" F. [; d6 Q% s E 4 Stabilized Ribosome Display for In Vitro Selection
0 F; Y* ?% i$ Y" S! c( U2 c 5 Eukaryotic Ribosome Display with In Situ DNA Recovery 75! Z, M* c2 r$ v7 C' ^! w( n
6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 875 l1 ? k+ Z4 J/ j! Q x j
7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101
( \: L) g8 ^. V" Q 8 cDNA Display: Rapid Stabilization of mRNA Display 113) E! A. I# P$ O& T( j' w
PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS
/ R- T! \- j0 F" Q% R% |* K3 W7 o 9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
" h! g, m' g/ E& X! }- q 10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display5 Y) l' ^6 x9 C% k, T
11 Evolution of Protein Stability Using Ribosome Display
! b: I# l$ W6 s7 O. I 12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
2 b0 q' k& r0 V3 N, |9 j 13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
4 D0 a. L! I" t7 G2 B 14 Peptide Screening Using PURE Ribosome Display9 k4 B, X% y% c" `# t
15 Rapid Selection of High-Affinity Binders Using Ribosome Display 2617 A7 W* N1 V \2 r$ j0 Q% {% h
16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287. ?0 `4 O% G5 C0 r, r
17 Identification of Candidate Vaccine Genes Using Ribosome Display 299
. C( s' P- i* O7 s) ~1 J5 h 18 Ribosome Display for the Selection of Sac7d Scaffolds 3157 L" r' q2 k( O6 R |$ C
PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS2 U. B* W5 N1 k- M; E3 @
19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335! j7 {, _4 d3 U: z9 Z) y$ O
20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349
1 s# t% y0 |' F; x- k 21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 367. H& V& q2 z8 j3 c
PART VI CASE STUDIES& D* m" S: Z i- n- M1 \
22 Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display6 P' ^8 {$ c! n
23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 403; m& Y4 j8 u% C- u6 P! s
Index 423
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