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本帖最后由 细胞海洋 于 2013-1-12 22:42 编辑
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Ribosome display and related technologies : methods and protocols " z2 f1 R7 e' W- `0 ~
edited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805)
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Contents
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Preface
* L& k# O! j% |4 _; W) V Contributors' K4 U0 C% ]3 H' C
PART I REVIEW7 |" E7 j7 u5 D5 y6 i; U
1 Ribosome Display: A Perspective( b f1 S: k6 m% L6 c9 X ^
PART II TRANSLATION EXTRACT PREPARATION- I( F7 }8 q( f( s
2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts& ?" E$ q$ w% c0 k7 w
PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS
0 A7 ^5 O& I9 K! Z7 g, A2 c7 O* I 3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 45
6 }# {9 B, ^ I. `( w+ s 4 Stabilized Ribosome Display for In Vitro Selection8 o8 c; K7 h1 t, t* M
5 Eukaryotic Ribosome Display with In Situ DNA Recovery 75
2 u" T- h% S0 ]9 x 6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 87; i, j) g4 S% @5 c
7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101 r. F- i' m" U: X% _. g
8 cDNA Display: Rapid Stabilization of mRNA Display 1134 z/ g+ e1 J$ g: I0 ^* v
PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS/ a& ]: H" Y. k: M5 X
9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis" s, S1 n1 N, N8 U* s' b# W7 a) ^
10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display D9 z1 A, o2 G' N1 E [
11 Evolution of Protein Stability Using Ribosome Display# [, s6 W% R2 \: v
12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries2 y- k6 M( X5 ?
13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
( P/ H1 H. e. p$ u' x$ I 14 Peptide Screening Using PURE Ribosome Display3 R& R3 S% @( X0 f; G% Z
15 Rapid Selection of High-Affinity Binders Using Ribosome Display 2610 G t' f. y5 t" ]1 ?4 E4 u5 f
16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287
7 n Y4 a! h0 [( z9 k$ p 17 Identification of Candidate Vaccine Genes Using Ribosome Display 299% U+ {! q: H6 ^) p$ i
18 Ribosome Display for the Selection of Sac7d Scaffolds 3153 N" f# T1 L* T1 f7 O6 H
PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS. y7 |+ @* `0 e4 d
19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335/ C- w3 [% V- X* s8 @1 h
20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349
+ c3 K- K9 L- L9 y; i4 ` 21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 367% `+ L: ^' c" d
PART VI CASE STUDIES$ ~5 B- U; ^& W8 ?
22 Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display
* y# H" \) ?) Z' S( J 23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 403
4 _1 D% ~8 Q" t: {' Z& w$ v$ U Index 423
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