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本帖最后由 细胞海洋 于 2013-5-3 11:03 编辑
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RNA and DNA Editing
. Z0 |' @" {, JMethods and Protocols
/ l; L4 e/ ?/ L* eSeries: Methods in Molecular Biology, Vol. 718
3 }& x+ p# }7 t# D3 n. J7 ~Aphasizhev, Ruslan (Ed.)
4 Z# x( `1 }$ L, I* b- t5 x) ~& O( P9 A2011, XIII, 275p. 57 illus., 1 illus. in color./ @' _ A' S' I
A product of Humana Press+ A+ E: I/ j( ]2 C: c5 y
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/ u; \- z' w6 R; w1 r/ t: G* j6 [( w' O+ d- A$ Z
Part I Uracil Insertion/Deletion RNA Edi ting
9 y5 V' D% ]' B# y% }# vin Mitochondrion of Trypanosoma brucei* h$ W) {" v t! N
1 Three-Dimensional Reconstruction of Trypanosoma brucei6 G) Z P' W& z; A
Editosomes Using Single-Particle Electron Microscopy . . . . . . . 3
$ A' G- B: Z( pH. Ulrich Göringer, Holger Stark, Cordula Böhm,
$ l1 b+ U% i4 R' E3 K. V4 r, wBjoern Sander, and Monika M. Golas
% v k# W% X% f/ v) \2 iCODA: RNAi-Based Inducible Knock-In System2 a. `- w6 v" Y
in Trypanosoma brucei . . 23( [! O) \# D2 @ |* |5 d- ]5 z4 H5 Q/ H
Gene-Errol Ringpis, Richard H. Lathrop, and Ruslan Aphasizhev
0 H' o/ r2 o; q3 X1 \( [Part II A denosine to Inosine RNA Edi ting* J8 b2 q+ A( }% l5 p
3 Perturbing A-to-I RNA Editing Using Genetics# S9 x5 l: o; u1 s2 |, E& s# m9 e
and Homologous Recombination . . 41' S1 a, p. C0 [9 W% t4 ]1 n
Cynthia J. Staber, Selena Gell, James E.C. Jepson, and Robert A. Reenan4 a+ s' a K* K& M7 r
4 Laser Microdissection and Pressure Catapulting of Single
8 {+ `3 t' r" X; L, |. c% gHuman Motor Neurons for RNA Editing Analysis . . . . . . 755 ^3 s3 M: H e" i t
Hui Sun, Aruna Raja, Mary A. O’Connell, Valerie Mann, Brendon Noble,' d0 j% @- r5 M& L5 T9 r \+ G9 d7 e
and Liam P. Keegan
/ E* P( B: R: |) M: P0 c5 Biochemical Identification of A-to-I RNA Editing Sites2 X5 n( b0 W$ i, R6 O6 q
by the Inosine Chemical Erasing (ICE) Method . . . . . . . . . . . . . . . . . . . . . 89
) P9 C! G) U& F* `Masayuki Sakurai and Tsutomu Suzuki
* _$ o3 X# i& @* I+ YPart III C ytidi ne to Uracil RNA and DNA Edi ting$ D4 q. K3 f" [" [ B$ J
6 Identifying mRNA Editing Deaminase Targets by RNA-Seq . . 1032 ?: f; [% O0 ?7 y% g
Brad R. Rosenberg, Scott Dewell, and F. Nina Papavasiliou
) l. l: p" T _ K) I7 Mouse and Other Rodent Models of C to U RNA Editing . . . . . . . . . . . . . 121) j) D" w$ r. @* x, Z9 b
Valerie Blanc and Nicholas O. Davidson
1 _4 w& m( ^$ G- T$ ?$ |8 In Vivo Analysis of RNA Editing in Plastids . . . . . . . . . . . 137
( _: `# v: r# c' `$ R: D* [( F0 k7 HStephanie Ruf and Ralph Bock
5 o* G9 B u P' V' F9 Identifying Specific Trans-Factors of RNA Editing in Plant
" N& V, M" E( q( p5 dMitochondria by Multiplex Single Base Extension Typing . . . . . . . . . . 151 w/ z! }. C2 V4 D+ {& t; ~, d
Mizuki Takenaka# q- g# K" |5 a$ q$ B
10 Complementation of Mutants in Plant Mitochondrial RNA* r" J- z; }6 ^# V1 u) E% i
Editing by Protoplast Transfection . . . . . . . . . . . . . . . . . . . . . . . . . . 1639 N( r3 N, d- w6 [3 {) V& h+ c
Mizuki Takenaka and Anja Zehrmann
) k* L! V( k1 F3 a11 A High-Throughput Assay for DNA Deaminases . . . . . . . . . . . . . . . . . . . . . . . 171
% o9 T& l- [" \: a4 bMeng Wang, Cristina Rada, and Michael S. Neuberger9 y/ T4 A6 }) i- r! I3 o
12 Biochemical Fractionation and Purification
+ [, F3 S4 e# ~: f2 e& U( Cof High-Molecular-Mass APOBEC3G Complexes . . . . . . . . . . . . . . . . . . . . . . . 185
9 D% C8 e' f5 W! Q3 @" }* PYa-Lin Chiu
+ O5 H2 D, e! P* `Part IV tRNA Edi ting and RNA Modi fications
' s% K+ }0 F7 m% ]/ A13 Analysis of tRNA Editing in Native and Synthetic Substrates . . . . . . . 209
7 d! m% _* ^. |' A6 rJessica L. Spears, Kirk W. Gaston, and Juan D. Alfonzo6 `4 [4 G7 h6 H" Q% Q X- H" [& [% h a
14 Post-transcriptional Modification of RNAs by Artificial Box
# `" F4 F% `; I# K0 E9 _* PH/ACA and Box C/D RNPs . . . . . . . . . . . 227: T' Z' x9 K( U' V
Chao Huang, John Karijolich, and Yi-Tao Yu
0 W& H: t3 R) t5 S* B, U) C2 s15 Functional Analysis of Noncoding RNAs in Trypanosomes:5 A) \" K% z; _* h' ^% `
RNA Walk, a Novel Approach to Study RNA–RNA Interactions& G4 A9 C( z3 A! t" S
Between Small RNA and Its Target . . 245
4 c Y- n% P1 b/ d$ `Chaim Wachtel and Shulamit Michaeli' K% m& q" z5 E, s [& U6 }
16 A Post-Labeling Approach for the Characterization
6 ] Q0 n7 N0 T( N0 N2 y, w) J5 Tand Quantification of RNA Modifications Based on
4 c9 }& z# t: b+ _4 s$ ISite-Directed Cleavage by DNAzymes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2596 f' z; y x) z
Madeleine Meusburger, Martin Hengesbach, and Mark Helm5 _. b1 i+ q- [0 u
Index . 271 _$ j6 O" ^& W+ p$ k! v
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