PDF电子书：Spectroscopic Methods of Analysis

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' {$ Y; p- Y! X7 B/ q+ A4 Z9 @Spectroscopic Methods of Analysis7 K2 K" K' R  }1 L! y
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Contents
! a6 }, F6 o: n' B/ Q" O) aPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v7 @8 E8 b; N/ r! K" q$ a
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix% [, x  N, H( r) s% Q- x! r$ |
1 Fluorescence Lifetime Imaging Comes of Age How to Do It and
7 i" h3 p; }- e& ^& |How to Interpret It. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1- E6 R: u6 A  Q# x8 r) S7 P
Yi-Chun Chen, Bryan Q. Spring, and Robert M. Clegg# n/ V/ A9 Z# L7 Z
2 Fo¨rster Resonance Energy Transfer and Trapping in Selected Systems:1 A7 M8 M* o" J* X0 P! i
Analysis by Monte-Carlo Simulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
2 v& h: N+ |0 R1 j+ \% O8 ]% jP. Bojarski, A. Synak, L. Kułak, S. Rangelowa-Jankowska,: d5 k6 H4 _8 p, X9 Y! ]
A. Kubicki, and B. Grobelna
0 S2 O9 r$ B: `# h3 N7 b* _9 v& M, D3 Molecular Organization of Polyene Antibiotic Amphotericin B Studied) W  n  M. c. N5 ~% m+ Y3 Z9 C7 q
by Means of Fluorescence Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
7 C0 I' s# S# SWieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko,. o: E5 N+ O  Q% J/ P9 X
Zygmunt Gryczynski, and Ignacy Gryczynski1 X% ]7 ^" X4 V/ \
4 Spectroscopic Probes of RNA Structure and Dynamics . . . . . . . . . . . . . . . . . . . . . . . 67- p2 l# x: S8 |6 l7 J( o
Kathleen B. Hall+ x$ Y; {# W0 i- m' x; F: I. J) Y
5 Fluorescence Methods to Study DNA Translocation and Unwinding8 S# a7 j2 R) s9 P9 v0 b
Kinetics by Nucleic Acid Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
' H4 U$ u1 I% f* S5 A  m8 D' y+ FChristopher J. Fischer, Eric J. Tomko, Colin G. Wu, and Timothy M. Lohman
; ^9 a/ c4 ?6 A9 M5 q3 |/ g& x6 Fluorescence Intensity, Anisotropy, and Transient Dynamic$ s3 X( f, T# a
Quenching Stopped-Flow Kinetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105; Z& n* M: k" ?  Q. t% T
Wlodek M. Bujalowski and Maria J. Jezewska" K8 Y" l5 {6 l" V) ]- @4 J
7 Using Structure–Function Constraints in FRET Studies
; @+ p3 i& x9 D1 ?; D! uof Large Macromolecular Complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135: Z5 M' h, I4 a' o- f- M4 f
Wlodek M. Bujalowski and Maria J. Jezewska( P& h' R9 l. |# Y0 y0 H, ^, b
8 Illuminating Allostery in Metal Sensing Transcriptional Regulators . . . . . . . . . . . . . 165; W+ f" Y8 u, y1 G" ^  E7 @  u
Nicholas E. Grossoehme and David P. Giedroc5 b4 G) Y! n7 E4 ~( N0 j/ I
9 Fluorescence-Based Biosensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
$ O: |  V0 l. Y! L2 jMaria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella,( @# i8 P; b- j7 h* K7 R
Claudio Pellecchia, and Sabato D’Auria
. {; {' `0 P& E* P% k+ v10 Metal-Enhanced Immunoassays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217
' \+ |* E3 L! W: T* F7 xIgnacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko,
3 V! ~8 O9 N$ g$ [4 _Pabak Sarkar, Julian Borejdo, Irina Akopova, and Zygmunt Gryczynski
' v: N2 [4 R( n: \3 \4 w  @. ~4 A/ C5 E11 Initial Stages of Angiosperm Greening Monitored by Low-Temperature
* g6 {" D7 V/ KFluorescence Spectra and Fluorescence Lifetimes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2316 s; l: _. B& X# H
Beata Mysliwa-Kurdziel, Anna Stecka, and Kazimierz Strzalka2 G' Q% |: r) C& f
12 Activation of the Mammalian Cells by Using Light-Sensitive Ion Channels . . . . . . 241
$ S* ^8 d7 j, j' M$ o1 X$ ?Mandy Siu Yu Lung, Paul Pilowsky, and Ewa M. Goldys
1 H: X) F3 c: k( _" O2 `5 Z+ k( p13 Detection of Specific Strains of Viable Bacterial Pathogens by Using RNA
4 u1 A" T/ u2 q  Y2 ]& L0 ^7 _Bead Assays and Flow Cytometry with 2100 Bioanalyzer . . . . . . . . . . . . . . . . . . . . . 253
6 I# S  t1 F2 y2 u9 w& j0 `" I5 QPhilip Butterworth, Henrique T.M.C.M. Baltar, Martin Kratzmeier,+ t# |9 E( ^+ B! b; f3 A% a% h, C
and Ewa M. Goldys* c  I7 K1 k1 K9 |: t) f
14 Photosynthetic Antenna Complex LHCII Studied with Novel
8 x; K1 M. N' K: U* ]. v/ MFluorescence Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263
; J$ @$ X: Z& F. E. C6 I" D; V1 zWieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski,
! S9 j, j( g) g" q& i" lZygmunt Gryczynski, and Ignacy Gryczynski
' e  X4 L; H, U- `7 [15 Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule
$ c6 |- v7 \2 H6 ~8 s. pFluorescence Spectroscopy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2716 }9 {# Q  U. [' v9 N
Goran Pljevaljcˇic′, Rae Robertson-Anderson, Edwin van der Schans,
  v0 a+ @. l+ j* J& }. W# Land David Millar- {& T4 A% b8 ?  e0 c8 D5 |
16 Single-Molecule Force Spectroscopy of Polycystic Kidney Disease Proteins . . . . . . 297
$ b/ l1 i7 D% U: k$ pLiang Ma, Meixiang Xu, and Andres F. Oberhauser$ Z1 g/ S1 z3 [0 Q/ l
17 Single Molecule Detection Approach to Muscle Study: Kinetics
# O3 C1 `5 ]* f7 Jof a Single Cross-Bridge During Contraction of Muscle . . . . . . . . . . . . . . . . . . . . . . 311
# d! K/ A6 W4 D& R6 TJulian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla,) [; K/ G% f2 j  E7 h3 ^6 Z6 O
Rafal Luchowski, Zygmunt Gryczynski, and Ignacy Gryczynski4 ?. z1 c1 x8 t7 z$ I
18 Single-Molecule Optical-Trapping Measurements with DNA Anchored0 G, _% Z8 M- P) Q1 r1 ?
to an Array of Gold Nanoposts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335
8 D3 g4 Z; ]. D9 SD. Hern Paik and Thomas T. Perkins
' t" x- M5 w% s) L5 ~2 z3 q19 Electro-optical Analysis of Macromolecular Structure and Dynamics. . . . . . . . . . . . 357
7 D4 x" t( a0 F, i# uDietmar Porschke
8 A/ }0 E* ^5 U: y) H20 Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen/ s& t3 C# K" z0 j  ^0 \% G
Intermediates in Cytochromes P450 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375! A2 j6 k7 o: \, F( I6 g, a3 @) O
I.G. Denisov, Y.V. Grinkova, and S.G. Sligar
. a3 ^" k  u5 iIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
& D  H6 G, ~- p! @ . . . . . . . . . . . . . . . . . . . . . . . 393
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