求教paraformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

未来之星

1 }7 c+ e6 h& z$ n8 A! R7 n0 S. Z1 ]% s( D: N* r
Carboxyfl uorescein succinimidyl ester (CFSE;0 S8 @' g# h" T1 p1 |/ D
Invitrogen)-labeled PBMC were cultured at3 v; j/ j( q+ w; x3 N$ |2 P
400 000 cells/well in a 24-well plate with cultured,- `# P% H! w# N
freshly thawed or paraformaldehyde (PFA)-fi xed1 U0 Y9 D% s- |  s1 u, V2 }
MSC at a ratio of MSC:PBMC of 1:3 and 1:9. T
6 j0 a2 v( w; m, p3 v3 \# \/ Rlymphocytes were stimulated using 0.4 μ g/mL antihuman+ n6 B7 j& ]2 k# G6 Q( i
CD3 and CD28 antibodies (clone HIT3a6 V: w$ i9 p- o: Z4 W, U
and CD28.2, respectively, from BD Pharmagen,+ n8 a: z' P! H. t
Mississauga, ON, Canada). T-cell proliferation was
# g$ E) ^' j5 s4 N0 B* Fdetermined 4 days later by fl ow cytometry analysis
0 V1 D* I' |' ?% v; N! U% s& ]of CFSE fl uorescence intensity.
  [- h. A# u1 n
" o& R/ |4 k, e# n* J5 _/ Z% j6 dparaformaldehyde (PFA)-fixed MSC 这是什么意思？记得共培养是用丝裂霉素处理？

cleargreat

丝裂霉素是处理PBMC的吧。

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回复 cleargreat 的帖子
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如果用丝裂霉素处理PBMC的话，怎么研究MSC对PBMC的增值抑制作用

cloudycity

这说的是PFA固定过的MSC吧···
! `- P7 Q1 n  L) Q但是一般灭活都是用丝裂霉素或是射线灭活，PFA还真没用过···

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回复 cloudycity 的帖子
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' g" ]9 c6 Q1 B" v4 n4 j" `+ AMSC为何要用PFA固定呢？