急求如何从病人的尿液样本中分离，提取尿液上皮细胞的protocol

innatestem

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) l& q( ~* o. t1 O8 N( [你好。谢谢你的分享，培养基的成分有哪些？你能分享一下吗4 X4 M( _* C) u7 S: e

时一清

Primary Medium： 5ml FBS，250μl P-S，100μl amphotericin B，REGM SingleQuot kit 每管 50μl，再 加入 DMEM/high glucose 和 Ham’s F12 nutrient 等量混合液加至总 50μl。 注：Store the medium in the dark at 4 °C and use it within 2 weeks.
! d- A+ Z2 g* ]+ }: V: cRE Proliferation Medium： 50ml REBM 培养基中加入 REGM SingleQuot kit 每管 50μl。 1 A0 j+ S. x- h' R
注：Store the medium in the dark at 4 °C and use it within 2 weeks. 5 u) ]. M, L* T# L0 D4 y* Z

, ]( D5 [* m( Q* W% J& T) m) E8 F& c建议你仔细看一下
* f/ K$ X/ l7 xGeneration of human induced pluripotent stem cells from urine samples.

niliu730323143

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, C6 P0 v4 \- A$ C2 k$ c: X/ iThe;primary;urine;cells;were;then;processed;and;cultured;in;urine;cell;medium;consisting;of;a;1:1;mixture;of;DMEM/F12;culture;medium;supplemented;with;10%;of;FBS;(FBS,;PAA),;0.1;mM;non-essential;amino;acids;(NEAA),;;1;mM;GlutaMAX;(Life;Technologies),;0.1;mM;β-mercaptoethanol;and;SingleQuot;Kit;CC-4127;REGM;(Lonza).! W$ k$ x' R% x2 P% X9 ?* L2 M
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参考文献：Generation of integration-free neural progenitor cells from cells in human urine。