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饶冠华

1: Cancer Sci. 2009 Aug;100(8):1397-402. Epub 2009 May 6.
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: u, _; h# n# K" [$ |1 v( Q2 mCancer stem cell-like SP cells have a high adhesion ability to the peritoneum in gastric carcinoma.
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Nishii T, Yashiro M, Shinto O, Sawada T, Ohira M, Hirakawa K.+ m3 ^1 r8 x" b2 Q* I; P
Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Abeno-ku, Osaka, Japan.; z* x1 m! }! Y- S) Y& X3 Z

0 m1 X4 P, w, ]; {; }, l3 m( K. _% |Cancer stem cells (CSCs) are considered to be responsible for cancer metastasis, but the evidence to conclusively prove this hypothesis remains uncertain. The side population (SP), as evaluated by a flow cytometric analysis using Hoechst 33342, has been known as CSC-rich population. The aim of this study was to clarify the characterization of the SP cells in peritoneal metastasis of gastric carcinoma. Gastric cancer cell lines OCUM-2M, OCUM-2D, and OCUM-2MD3 (a daughter cell line with high potential for peritoneal metastasis) were used. We isolated SP cells from OCUM-2M and OCUM-2D using flow cytometry. Serial sorting was performed three times to enrich SP cells, and they were designated as OCUM-2M/SP and OCUM-2D/SP cells. Flow cytometric analysis showed 0.46%, 0.29%, 5.24%, 6.49%, and 11.3% of the SP cells to be found in OCUM-2M, OCUM-2D, OCUM-2MD3, OCUM-2M/SP, and OCUM-2D/SP cells, respectively. The intraperitoneal inoculation of SP cells and OCUM-2MD3 cells produced peritoneal metastasis, but parent cells did not. The adhesion ability of SP and OCUM-2MD3 cells was significantly high in comparison to that of parent cells. The expression level of adhesion molecules alpha2-, alpha5-, beta3-, and beta5-integrin, and CD44, was high in SP cells compared to parent cells. The expression of stemness markers, Oct3/4 and Sox2, increased in the SP-cell-injected tumors. These findings suggested that CSC-like SP cells expressing alpha2-, alpha5-, beta3-, and beta5-integrin, and CD44, may play an important role for peritoneal metastasis in gastric carcinoma. Oct3/4 and Sox2 may be associated with CSC in gastric cancer.
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PMID: 19493275 & B- B2 t6 @/ l4 t9 q

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hualin840518

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5 a4 ]4 T2 ]: Y7 w9 oCancer stem cell-like SP cells have a high adhesion ability to the peritoneum in gastric carcinoma

饶冠华

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franklinhg

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