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- 积分
- 264
- 威望
- 264
- 包包
- 858
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O& n) |& R% u# {6 x" y' K/ n
(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g2 V6 {/ T6 }6 k) c$ x Q/ q* v
MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),/ x; I; r! f, {
4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;: j9 y5 v& g7 C; I0 ]
Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.) H" ?3 m- ~8 z( Y
G6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g
. S) s& e6 S. bpenicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate% `) W9 a" o" n
(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of+ @; w1 L7 y7 l6 A ?6 s& l; ?
Milli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L. x |% a. u1 ]0 K4 s' r
with Milli-Q water. |
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发表于 2009-9-21 09:25
发表于 2009-9-26 07:48
