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欢迎加入讨论,如果你做PGC的培养,请注意她的时间段,小鼠是胚胎期E13.5以前称PGC,人胚胎期E5-9week称PGC,此后停留在G1/G0的Mitosis arrest期,直到出生后才开始恢复有丝分裂" p' s% E& p' ^6 m2 A1 D
4 U! |9 s) o9 m4 n# I如果你刚接触,我推荐你看看下面的文章
1 h, @- ?9 p5 a2 |/ G+ M1992 long-term proliferation of mouse primordial germ cells in culture+ o/ O. J0 r s- v
1993 Proliferation of Mouse Primordial Germ Cells in Vitro A Key Role for cAMP8 v/ o: W5 ~! ~0 P
1994 Role of leukemia inhibitory factor and its receptor in mouse primordial germ cell growth
$ y+ d* S8 ~- S' l8 X: }5 ^$ ]" g1995 Purification of Mouse Primordial Germ Cells by MiniMACS Magnetic Separation System
# _( x9 X0 F+ ~! |, `( C5 ~1995 Retinoic Acid Is a Potent Growth Activator of Mouse Primordial Germ Cells in Vitro
1 P+ T0 o1 @( G# Y! s2 @1996 Autonomous Regulation of Proliferation and Growth Arrest in Mouse Primordial Germ Cells Studied by Mixed and Clonal Cultures9 `6 Y# Z' ~6 g3 j1 u! K C
1998 In vitro adhesiveness of mouse primordial germ cells to cellular and extracellular matrix component substrata- i9 R5 V, J, b, k* }
1998 Role of Fibroblast Growth Factors and Their Receptors in Mouse Primordial Germ Cell Growth# ?: q2 G% g1 }6 A( z
2000 A Role for E-cadherin in Mouse Primordial Germ Cell Development
& \, t. Z# b' g: d/ V A" ]2000 Regulation of primordial germ cell development in the mouse3 g7 k# U; I8 n& g+ k
2001 The allocation and differentiation of mouse primordial germ cells
1 Q9 R5 H& g7 w! i/ O/ v& K3 R2001 Twenty years of research on primordial germ cells
' Q/ Z) u t; W. Z+ ` F2003 Developmental fate of embryonic germ cells (EGCs), in vivo and in vitro5 v( W( @+ p4 |
2003 Primordial germ cells in the mouse0 p- ]& V( Y9 d- n) g
2003 Purification of mouse primordial germ cells by Nycodenz3 k$ \' ^# J/ O& T
2004 Autocrine and Paracrine Mechanisms Regulating Primordial Germ Cell Proliferation
+ u( ~: h$ `3 _2004 BMP signaling mediated by ALK2 in the visceral endoderm is necessary for the generation of primordial germ cells in the mouse embryo% y+ J5 u* z& L0 ?
2004 Experimental approaches to the study of primordial germ cell lineage and proliferation" f. g8 J; {" k9 p( B3 T. |
2004 Oct4 is required for primordial germ cell survival
& O. z2 b, }+ m: k0 P8 @2004 Regulatory role of BMP2 and BMP7 in spermatogonia and Sertoli cell proliferation in the immature mouse.
$ M& K- K# \6 z2 {( `2005 Growth factors sustain primordial germ cell survival, proliferation and entering into meiosis in the absence of somatic cells
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6 Z' V% R# q3 l+ v' s. g; R; Z+ |上面全部是小鼠的PGC分离,培养,鉴定和信号调节的文章,不全,但比较有用.你可以挑你感兴趣的看 |
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发表于 2010-3-8 17:47
发表于 2016-2-25 14:56
