请教精原干细胞相关问题

milklecherry

大家好，本人想请教：精原干细胞的研究前景如何？人精原干细胞的体外分离培养、纯化的难度是不是很大？存在哪些问题？目前有没有建立人的精原干细胞系？人精原干细胞诱导分化为精子样细胞难度大不大？本人刚涉及此领域，对此不大了解，敬请各位大侠指导！

yiwuya

研究前景如何？4 a, N1 c$ w# q- Y  B; W
Male germline stem cells: from mice to men. Brinster RL. Science. 2007 Apr 20;316(5823):404-5. Review.
: m$ C  ?. Q/ K. y7 {; k! xGerm cell research: a personal perspective. Yanagimachi R. Biol Reprod. 2009 Feb;80(2):204-18. Epub 2008 Dec 17. Review) [% Q9 o" g( d/ D/ x
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目前有没有建立人的精原干细胞系？! _8 y9 [: q% n2 Q- _4 O4 H
Propagation of human spermatogonial stem cells in vitro. Sadri-Ardekani H, Mizrak SC, van Daalen SK, Korver CM, Roepers-Gajadien HL, Koruji M, Hovingh S, de Reijke TM, de la Rosette JJ, van der Veen F, de Rooij DG, Repping S, van Pelt AM.JAMA. 2009 Nov 18;302(19):2127-34.8 B0 T8 {9 A; G( }  g  ^
with erratum in: JAMA. 2010 Feb 3;303(5):422.
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- Y6 R" G9 n5 c. M  M: f/ r: M) m人精原干细胞诱导分化为精子样细胞难度大不大？
* C* }% f8 W" K8 K% v8 o6 r4 Z3 ^+ aBelow is an unavailable cell line and unrepeatable work.
' j. D$ z9 w( m' B! v% a5 X2 ~, E( tGeneration and in vitro differentiation of a spermatogonial cell line." G% Q% B+ y* r, D; ?
Feng LX, Chen Y, Dettin L, Pera RA, Herr JC, Goldberg E, Dym M.Science. 2002 Jul 19;297(5580):392-5. Epub 2002 Jun 20.
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