PDF电子书：PCR based GENE Protocols 经典书籍

shinejesse

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PCR 应该是分子生物学最基本的实验手段，但并不是每个实验都有一帆风顺的，基于PCR的实验越来越多，SSCP，定点突变，* s, U  Y* J2 x; K
等 PCR进行基因沉默的制备。比较实用的基因手段。大家根据需要选择下载，希望有所帮忙。: ?( R( l# ]" @
Preface .............................................................................................................v
) v, a; F% M) q( f* r, XContributors .....................................................................................................xi) O6 Q- m) P0 R, q6 H
PART I. PERFORMING AND OPTIMIZING PCR4 k" o/ H8 t, X  r
1 Polymerase Chain Reaction: Basic Principles and Routine Practice
# S* q6 Q/ R3 O3 |0 E" qLori A. Kolmodin and David E. Birch .................................................. 3
* m" y6 P% i" p( v: c2 Computer Programs for PCR Primer Design and Analysis$ F4 |3 K0 m0 |2 Y; h3 \; k
Bing-Yuan Chen, Harry W. Janes, and Steve Chen ........................ 192 ?" Z8 z, p2 {) b& u. u' b
3 Single-Step PCR Optimization
) M' X- `4 x5 k* k5 Q) QUsing Touchdown and Stepdown PCR Programming
% C: X4 S' H$ \" J, c6 QKenneth H. Roux .................................................................................. 310 x% v5 f! b2 [1 h: O; t: n
4 XL PCR Amplification of Long Targets from Genomic DNA
7 t# l( z. E- K: R0 ]: cLori A. Kolmodin .................................................................................. 37
* t; h+ b' a4 W5 X$ \! U% F5 Coupled One-Step Reverse Transcription and Polymerase Chain
, u3 y5 a/ I$ Q& j" sReaction Procedure for Cloning Large cDNA Fragments
/ h6 X; T  R1 L- JJyrki T. Aatsinki ................................................................................... 53
. X0 Z+ F" X' e2 N1 {/ H6 Long Distance Reverse-Transcription PCR. z! H# C: a1 v  y# Y8 h7 q
Volker Thiel, Jens Herold, and Stuart G. Siddell ............................. 59
& X1 e. L7 N  T, D* t: V7 Increasing PCR Sensitivity for Amplification( |9 p0 ]1 b$ F2 }
from Paraffin-Embedded Tissues2 O4 r  X' I, Q) o; z5 o* _! |- t
Abebe Akalu and Juergen K. V. Reichardt ....................................... 67
# M0 G5 ], n$ Z. f. f1 Q8 GC-Rich Template Amplification by Inverse PCR:6 g0 F. t! @+ s* ~- D# [0 ^
DNA Polymerase and Solvent Effects
8 e8 X" [: w0 S9 r4 ?Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez,
1 E, `+ N( t9 Y! |8 Uand Jean Dusart............................................................................... 75& p3 ?) V6 ]$ j; C/ @& q! L
9 PCR Procedure for the Isolation of Trinucleotide Repeats1 Y7 Q! H$ C6 x$ W; ~( S
Teruaki Tozaki ...................................................................................... 81
1 J. E$ r! ?1 g1 g5 M10 Methylation-Specific PCR
7 A& p# d" o! g% {- RHaruhiko Ohashi .................................................................................. 91! ~" x$ J9 S7 [9 I2 `& ~& i
11 Direct Cloning of Full-Length Cell Differentially Expressed Genes! G$ t$ C0 v9 L- Z/ Q& w$ V( f
by Multiple Rounds of Subtractive Hybridization
4 T% B, A& G* ?Based on Long-Distance PCR and Magnetic Beads5 ]2 S; b: T9 o- j" `' h7 j
Xin Huang, Zhenglong Yuan, and Xuetao Cao ................................ 99
5 O1 j3 ]$ U8 @) a. I+ xPART II. CLONING PCR PRODUCTS$ o  s7 [. @& r4 k2 a( H
12 Cloning PCR Products: An Overview
4 g$ b7 ]6 u0 U: C/ m$ o8 bBaotai Guo and Yuping Bi ................................................................ 1114 {* u, r1 w, O9 Y( Z
13 Using T4 DNA Polymerase to Generate Clonable PCR Products8 E6 {4 B( N  R
Kai Wang ............................................................................................. 121* P- d1 x1 }& J* m& w
14 Enzyme-Free Cloning of PCR Products
8 w2 z# O) H' Y+ Aand Fusion Protein Expression
6 h  j! S7 F3 LBrett A. Neilan and Daniel Tillett ..................................................... 125
: |& g, f% u2 e15 Directional Restriction Site-Free Insertion of PCR Products, v+ }5 i5 f0 K( x" W$ e% q2 E
into Vectors% l( z, J& I9 x& V
Guo Jun Chen .................................................................................... 133
5 e% P8 r6 p7 v% S! D16 Autosticky PCR:
9 S$ \5 j# \. P7 q" u. G- sDirectional Cloning of PCR Products with Preformed 5' Overhangs
% C4 M+ D# n5 o& G/ k  d3 iJózsef Gál and Miklós Kálmán......................................................... 141) q, [$ l2 {3 F$ N; ~
17 A Rapid and Simple Procedure for Direct Cloning% z; B1 ~, G9 q' y3 X+ M3 _
of PCR Products into Baculoviruses
, g5 |) t5 }! e+ V$ b" r2 pTamara S. Gritsun, Michael V. Mikhailov,
+ u2 X4 h# H  t6 d! w8 y" J" gand Ernest A. Gould ...................................................................... 153+ O$ v; M* m; s/ ^4 m7 e
PART III. MUTAGENESIS AND RECOMBINATION
0 @, Y! r4 ]: }6 M- t0 v) ?$ F) c18 PCR Approaches to DNA Mutagenesis and Recombination:
3 X: v2 `' H, k* D! lAn Overview& B5 S/ C* j1 i; r
Binzhang Shen ................................................................................... 167& I$ s4 w: B* d# g& f
19 In-Frame Cloning of Synthetic Genes Using PCR Inserts
5 v% z" R, P# j8 n; P' Z  D/ YJames C. Pierce ................................................................................. 175
; V3 }) O1 [7 a( n" ?( L6 g, t20 Megaprimer PCR# w) y4 k0 I; w2 O: k( Z: f& E
Sailen Barik ........................................................................................ 1893 r0 F" j1 V0 ~! S
21 PCR-Mediated Recombination:1 D; z# Q  K/ g! J
A General Method Applied to Construct Chimeric Infectious& Y: U2 ^0 o: C
Molecular Clones
1 p9 @1 Y0 x" t% u) T; O! f! S8 X; E. wGuowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran,
" P5 o+ [4 a# T! band Harold Burger ......................................................................... 197
/ s# l' q; _. w% Q0 W  z5 u22 PCR Method for Generating Multiple Mutations at Adjacent Sites
4 ]' d3 g" {/ ZJiri Adamec ......................................................................................... 207
' H) t# j) i+ _7 x# r) ?23 A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing
9 q) A  {  X, J4 o0 z' ERepeat Expansions into CAG-Repeat Containing Genes
3 h$ R' m- Z: F) ]( ?. VFranco Laccone ................................................................................. 217
9 M! M0 W% O4 n9 I24 PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
) o# U$ N0 Q, w+ @- G& [; l$ }5 JDario E. Lehoux and Roger C. Levesque ....................................... 2251 E# B( m- @5 t* L: }+ u- \# {3 a
25 Staggered Extension Process (StEP) In Vitro Recombination2 d+ j% X3 W" r! j2 A: _! D
Anna Marie Aguinaldo and Frances Arnold ................................... 235
/ N. i- E7 b* J$ C! l: Y- D26 Random Mutagenesis by Whole-Plasmid PCR Amplification
2 N7 a1 X2 \; N! f! ^! i0 XDonghak Kim and F. Peter Guengerich .......................................... 241
. Z0 Z- y" M" V6 n8 x& T4 cPART IV. CLONING UNKNOWN NEIGHBORING DNA
7 X8 k& G; o; K27 PCR-Based Strategies to Clone Unknown DNA Regions+ a4 W; B! D' _/ v4 X" ^
from Known Foreign Integrants: An Overview( M1 K, J* U1 s$ p! u' G0 L/ B7 d6 [# ~
Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo ................ 249
; O: n0 f5 t7 u28 Long Distance Vectorette PCR (LDV PCR)
2 E' H6 T* I: K1 a& k! ]( FJames A. L. Fenton, Guy Pratt, and Gareth J. Morgan ................. 275' t+ o5 R$ A7 B0 i$ U( s
29 Nonspecific, Nested Suppression PCR Method1 E' P" u0 B, D' G7 @3 ~
for Isolation of Unknown Flanking DNA (“Cold-Start Method”)7 D. w5 c# W, E$ h& ?0 j$ m: j3 p
Michael Lardelli .................................................................................. 285% G/ m( [* _) T) \7 m. a' \* w2 n: \% I
30 Inverse PCR: cDNA Cloning
# d3 c9 J% z5 q5 aSheng-He Huang ................................................................................ 293  b& ]6 K/ I% n; \; u- j4 C
31 Inverse PCR: Genomic DNA Cloning
4 }& u3 d1 \. w2 UAmbrose Y. Jong, Anna T’ang, De-Pei Liu,
4 E9 P* g) F8 P  ^8 o4 @/ Hand Sheng-He Huang .................................................................... 301
' t) e* ^; D% u32 Gene Cloning and Expression Profiling by Rapid Amplification. O3 L# G1 i, a9 t. W$ c+ Z" h" G) J
of Gene Inserts with Universal Vector Primers
+ L7 o2 t9 u8 o" U. y3 H  PSheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong .............. 309
# b+ X% q, }, Y2 q  y) G0 u33 The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions& ~* r2 n+ ]7 E+ Z% F$ h. H
by Inverse PCR( ^) \4 a: L( b; @/ {
Vincent J. J. Martin and William W. Mohn ...................................... 315
. V# s) \+ Z7 r  h34 Rapid Amplification of Genomic DNA Sequences Tagged! @4 H4 i0 n- X9 F) w8 v: R4 ]
by Insertional Mutagenesis
# D4 e( h/ Z" ~Martina Celerin and Kristin T. Chun ................................................ 325
+ q3 |! g* ?8 j* H35 Isolation of Large Terminal Sequences of BAC Inserts Based
$ O* }. q7 [3 D4 S: o# }on Double-Restriction-Enzyme Digestion Followed1 O8 B! C8 u. ~3 F
by Anchored PCR
% Q: l2 z0 x  u% LZhong-Nan Yang and T. Erik Mirkov ............................................... 337
7 i* w$ k* q/ B( Y% s; q) t36 A “Step Down” PCR-Based Technique for Walking
& [; P" V( b) v2 L, a* W6 xInto and the Subsequent Direct Sequence Analysis
, _$ L, E  p1 D( j$ U- ]* gof Flanking Genomic DNA
. L  d& o) }. E" wZiguo Zhang and Sarah Jane Gurr .................................................. 3436 r& I6 ~1 t9 q' F3 }" n& y
PART V. LIBRARY CONSTRUCTION AND SCREENING
/ L2 D3 U+ v! w! y. [37 Use of PCR in Library Screening: An Overview( v# Q) e6 N, {8 ~4 d- c* Z/ |
Jinbao Zhu .......................................................................................... 353" m. t9 s% f4 T* y
38 Cloning of Homologous Genes by Gene-Capture PCR
+ q* ]9 e3 V8 Z3 N  yRenato Mastrangeli and Silvia Donini ............................................. 359
+ P( E% B( Z" ^  h& L3 N; e39 Rapid and Nonradioactive Screening of Recombinant Libraries by PCR
# J+ a) G8 J, H* S5 q. w! k' yMichael W. King ................................................................................. 377
9 a4 {6 r2 N3 B8 [& s40 Rapid cDNA Cloning by PCR Screening (RC-PCR)1 w2 z( \4 B; x8 Q/ D# w  v3 s
Toru Takumi ....................................................................................... 385
& q" E  a* l" o) O( j7 [" w41 Generation and PCR Screening of Bacteriophage λ Sublibraries5 @  |' \/ [, |$ k1 c) J
Enriched for Rare Clones (the “Sublibrary Method”)
- Y. B) |% O# f% c9 X% LMichael Lardelli .................................................................................. 391$ [. c9 f: \# G- z
42 PCR-Based Screening for Bacterial Artificial Chromosome Libraries
. @; p. R* {9 u8 P5 n, G% f# P. _Yuji Yasukochi ................................................................................... 401
# W. ^8 M9 V" K: U7 r43 A 384-Well Microtiter-Plate-Based Template Preparation
/ n! x7 }# a" T2 l% z$ a3 v0 I3 `and Sequencing Method" |$ s: p. f5 S2 |5 M7 w9 t
Lei He and Kai Wang ......................................................................... 411( j& W! m" V7 ?) l3 z$ m
44 A Microtiter-Plate-Based High Throughput PCR Product* R/ ^, C: k+ a# z
Purification Method
7 k  }) k8 R2 t9 VRyan Smith and Kai Wang ................................................................ 417" b" C/ T3 F" j) g
Index ............................................................................................................ 423* y" E) ^! H! Y3 I, H

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