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- 积分
- 164
- 威望
- 164
- 包包
- 1030
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Our electron microscopy showed that although the mouse oocyte5 b" a# F' i- o+ X7 k- U
had a wide perivitelline space and few short cell junctions on the
F' t% L# S5 H Hegg surface, the rabbit oocyte showed no perivitelline space but numerous( a$ V/ ~% _( h/ a! R5 I" C
long gap junctions that projected deeply into the egg cortex.1 t6 X* P) @ b/ ~
As a matter of our experience, it was much more difficult and took
- C, h/ c; e4 R* X, H( f' imuch longer time pipetting to remove cumulus cells from rabbit than$ q" ]: ~" k1 ?& ^
from mouse oocytes. Shear stress generated with use of a rotating
0 g6 a' C4 n# O% Rwall vessel or by pipetting induced stress-activated protein kinase/
% o( ]! N1 R ]5 z& }3 Q1 Ojun kinase phosphorylation that caused apoptosis in mouse embryos) X1 h0 G& V M; l+ F# V( X z3 k
(41, 42). It is therefore deduced that because the deep-set junctional
; l/ @5 x6 o( n r( H/ kcomplexes firmly anchored the oocyte and corona cells, although the7 j- y1 S& E' i3 O
removal of corona cells damaged plasma membrane of rabbit oocytes,4 R2 [3 l7 e4 Y; ~7 _; f9 x, @
leading to their degeneration or lysis, the long-time intensive
- K9 e r; H! g$ S* r9 b3 \pipetting during cumulus denudation induced oocyte apoptosis and3 O" D- I- ^2 y: r* A8 A
inability to mature in vitro.1 [( {1 ~6 }7 `
请问黄色字幕这两句怎么翻译呢?谢谢
! h& ^3 L; L& l% B" f) j3 ~3 V: u' Z& l. F
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