PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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) k* g6 O! @3 H& ^" q0 L& iPDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press4 g( {, q. B4 O+ l# Y
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主要内容如下：
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Contents- j7 X0 W4 D6 ~% f2 e
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii7 o# N6 p8 h5 ]
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi
+ B" p/ [' O# H  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1
- p7 V0 M; b2 e" x0 K# @  bChristina Philippeos, Robin D. Hughes, Anil Dhawan,
, S: d  x; `- e2 xand Ragai R. Mitry6 U- ]7 j  M) a- D6 O
  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate   r. v) z) l- r% y# X  i
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15
5 }3 ~3 n; H6 p3 xLing Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn: t" n* F/ p" t' z2 B0 \
  3  Isolation, Cultivation, and Application of Human Alveolar / L" P2 D' q% e0 V$ F: c1 U
Epithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
  n& S" F+ G: A4 N5 e! \  lNicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, 3 N- Y! W5 d0 c
Hanno Huwer, and Claus-Michael Lehr
& [- E. B, b4 l2 S# C. V3 n4 B% j  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43" x. G( j, |/ B1 t& p
Peyman Björklund and Per Hellman/ ?) H! i7 E: U& G  x, h( V# F
  5  Functional Analysis of Human Islets of Langerhans Maintained
7 Q0 w4 `) J: j7 u% K# kin Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55
8 z- R' s% a8 J; w# PShanta J. Persaud, Bo Liu, and Peter M. Jones ) y3 t6 n- H  c
  6  Conversion of Non-endocrine Human Pancreatic Cells " B, w7 ^/ V1 [4 W# ]
to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73# e; I3 [# l: O4 m
Min Zhao and Guo Cai Huang/ d9 N+ P( y; v2 t9 U
  7  Evaluation of Cytochrome P450 Activities in Human " O6 b; W+ X" b. {4 V& o
Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  87
/ C* ~3 X3 t; A) p8 wMaría José Gómez-Lechón, Agustín Lahoz, José V. Castell,
" }# P' ^9 [' A& iand María Teresa Donato
6 o4 t9 j7 @% r* ?: m7 Y% s  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99
$ z+ ?0 f) C6 C$ MDaniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, 5 p! i: L# o0 \# M, s9 @
Peter Büchler, Michael Schoenberg, Jörg Kleeff,
9 l/ ?( Y8 w# P4 ?. FWolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy,
8 F* ~) r$ Z/ n$ q5 t. D7 |! GJan Hengstler, and Andreas K. Nussler
$ w' r" ^. Y: W3 q8 _  9  Preclinical Testing of Virotherapeutics for Primary and Secondary
9 v% Q! _' \/ s4 b6 d* C' sTumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121
! {4 k( Q; J4 |Martina Zimmermann, Timo Weiland, Michael Bitzer, % H. y# X. F& u
and Ulrich M. Lauer8 S* g4 D; ~2 `# @. {
10  Isolation and Functional Studies of Human Fetal Gastric 4 a0 l" U4 q# G+ a( x
Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
  X1 u% [) q: FPierre Chailler, Jean-François Beaulieu, and Daniel Ménard
5 t2 e! ~1 c( Z; s; {11  Isolation, Characterization, and Culture of Normal Human
7 o0 ~) ]4 M( v" b6 C& GIntestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157
7 D7 k# G# {0 h! j2 F) {# nJean-François Beaulieu and Daniel Ménard6 H2 A, [# h4 v7 [4 J
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12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells ( B" D7 X0 C; w
and Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175  a) o. P" k+ j) J" R* Z
Claire C. Sharpe and Mark E.C. Dockrell
3 I8 {9 v( t! S/ i13  Glomerular Epithelial and Mesangial Cell Culture & r2 y, C+ [6 S: ?7 O
and Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187
) i2 F/ \0 y+ n  g: f" M0 f- T% p0 zHeather M. Wilson and Keith N. Stewart
0 n4 }$ ^( e# r; o* t14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203
9 \6 X4 y$ X, D2 ?9 F* ?6 KKirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried
% [, A* G$ J' m6 Q$ p' q; e5 }15  Primary Culture of Human Adipocyte Precursor Cells: * I2 u5 c5 ?1 S# e
Expansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215
, `* U8 b# f1 hThomas Skurk and Hans Hauner  n8 v, m$ a5 \# d- R3 s/ k
16  Primary Culture of Ovarian Cells for Research on Cell Interactions 1 z( v, D+ `' J0 V7 G: n! J
in the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227
' z: k' {$ F- wJerzy F. Galas/ s. q# Y) p! |$ w0 M5 X7 `& o( h
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251  C/ b: ]  U4 E: v; c
Diane Proudfoot and Catherine Shanahan
. A+ F7 s7 r9 O8 A18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265
9 ~( @& u/ R4 z' L( a! u, uRichard C.M. Siow+ C! j' M0 `' z4 ]( n. `( d/ k
19  Human Peripheral Blood Mononuclear Cell Culture for Flow
& ?) }0 K; T% x: s4 [+ r0 ?1 rCytometric Analysis of Phosphorylated Mitogen-Activated
( k! p; e2 Q$ e/ ~Protein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275
1 t$ J! I6 |5 O& TAthanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou,
: ~$ M4 W6 Y5 u- E- m  Aand Dimitrios P. Bogdanos
9 B/ A! M, U9 ?4 Y1 p) m8 k3 r20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287' a1 ]/ p6 g! j9 y& l
Haibin Su, Maria Serena Longhi, Pengyun Wang, & u5 Z, T% y; t3 ]# E6 v+ L. l" L. `
Diego Vergani, and Yun Ma5 F2 x& I/ E% ~! [' R
21  Human Chondrocyte Cultures as Models of Cartilage-Specific
& }# V9 J  R, p$ PGene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3019 c6 U# T4 r# Y" h1 u" l0 |' f5 b
Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem,
4 d1 q3 l; p! l$ hKo Hashimoto, Darren A. Plumb, and Mary B. Goldring% l* D0 l) m8 b0 C) q0 O. h" n
22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3371 {- b) r9 Q# {- X  P( \7 m
Alison Gartland, Robin M.H. Rumney, Jane P. Dillon,
6 n' W( r/ e8 t3 M/ Cand James A. Gallagher
% y; v+ Y# K6 h/ d4 s9 E6 D5 }23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  3573 F- H8 v1 l9 N
Ankita Agrawal, James A. Gallagher, and Alison Gartland' s! H- M  r  ^) z; p4 G2 J
24  Effects of Temperature Generated from the Holmium: YAG Laser 8 p! `7 j, ?1 N7 V+ N2 A
on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377; s8 r! _( c  c9 M
Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, - p& ?; E. m5 D% n/ f
Ian D. McCarthy, and Al-Shymaa M. Hafez
, G$ O3 }  I5 r( i$ l- v25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  385
* D; l/ e, Z% O/ [% L- WAhlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia
# j# F, ]$ {/ ?- ^26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393
( Z4 d+ w' n2 R, k, n! m0 i2 oRobert Thomas and Elizabeth Ratcliffe0 W- ~5 N1 A+ Y; b: s& L
27  Culturing and Differentiating Human Mesenchymal Stem Cells
6 u7 n) o$ i  Pfor Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  407
! j/ ]; Z, u8 I( p$ ~William R. Otto and Catherine E. Sarraf
/ K/ \: A& {9 f  w& L5 T2 d9 GIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  4278 v" `1 x* E! G' k5 p, T

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