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- 积分
- 264
- 威望
- 264
- 包包
- 858
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O
2 c+ t6 y# p b; e( [(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g" F5 W/ i6 ?& p1 X3 [* D; l
MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),
2 _" X% v& d7 Z. ?4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;
# o m- ]) O( P& @" CSigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.; X! @ a" l* ~7 Z
G6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g" k: A# Z5 R B* j/ L! M& O
penicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate
8 W; g" q( j1 l0 x, j% w(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of, o: m, C; p6 }3 h) `1 @" H6 y
Milli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L, n6 @( q7 O$ z! J$ ~9 r
with Milli-Q water. |
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总评分: 威望 + 10
包包 + 10
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发表于 2009-9-21 09:25
发表于 2009-9-26 07:48
