M2培养液

POPOLD

M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O, B' T: [* e# j; V, i. j& {
(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g
& {+ U) J9 c8 t( }! y) N) Z5 D1 gMgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),
+ f0 _3 a; D# S3 Z! M& G' \4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900;) \- R! O' D# G" ^1 j2 N
Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.
  [( Y) Q( P2 Q+ {6 P9 J% EG6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g
: v0 ~9 `. V/ l- S# Dpenicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate! d2 w% w, D' f( |3 J8 k
(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of8 \4 N2 t3 E  s4 U7 ^( @
Milli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L
& ~: }2 Z/ m# p$ @: nwith Milli-Q water.

zpzp0312

谢谢！~~~

quixote1983

还有编号，太职业了

POPOLD

您的 满意 ，是版主最大的希望，还可亲临指导

jayhaoli01

谢谢！

hualin840518

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8 x7 C9 V7 t- v- l% K    来源是那篇文章
* [; k* ~6 E+ |1 b: Z5 z) N谢谢！

edisonlou

谢谢

llingzu

谢谢

基拉

谢谢

POPOLD

回复 12# hualin840518 ) r1 @: h* {* M$ |

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