有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章' I. N( n/ t* v5 R# O+ ~
2002 Lactate inhibits germ cell apoptosis in the human testis
& f# J* s; T# D" ~8 _2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture
5 \; k8 W! @; ^; t% R( n2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal/ z% m- F9 ]9 W
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells* w8 k0 u% X+ W( \& {0 i+ q
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro
, Z7 X; t9 T7 A' }. N6 b* d0 }: A9 @8 U2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture" X( i$ S6 [% e1 w+ @9 O0 a! r
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis2 M/ c4 E0 Q6 C
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture# e$ e# `8 w' b/ j2 @; t
2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse/ X. V; L0 b. d  V/ q
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines
) j4 M7 b( r8 i2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells8 C. q3 s$ f8 p% q# a2 A1 R. Q
2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells: G7 W8 {# _- B' B" z
2005 Anchorage-independent growth of mouse male germline stem cells in vitro" u8 W2 C) ^0 p  y4 ?
2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor8 H' Q1 g/ ]2 b8 Y& l3 X( ~& l1 H! b/ F
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
5 [. b7 k1 \& J# _+ }+ l2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment* E+ T; `: u# ?4 Y6 W
2005 Isolation of male germ-line stem cells; influence of GDNF* B# Q* \0 P( I5 r
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
+ T; E1 P4 T8 Y6 f$ u* R2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
% g- Q9 `9 l8 T' j, x2006 In vitro propagation of spermatogonial stem cells from KM mice4 i3 p3 J) ~0 R1 H( R( {$ [  g& ^
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上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的