有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章: j0 W) h9 s/ o/ C8 i6 e  ^
2002 Lactate inhibits germ cell apoptosis in the human testis
/ n3 P; _! \1 A8 ]% F$ ^2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture
- `& }0 L( n2 s' \6 n2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal
0 T4 V, Z% ]7 O9 K4 Q+ Q, ?2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
7 R; O* ~9 ^9 |9 Y, s8 z2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro
. E" n, S0 V5 V' B1 K. Z2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture- y' c3 c9 l% G3 D! I8 u
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis  c- Z" l2 x) b
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
) S/ C1 @9 P9 o8 D+ x, I2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse
9 v' g' U. @5 ~- ]( ]6 a" l! s4 \2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines3 \9 `( }3 \6 B9 \4 ~
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells9 Q+ G2 v0 \; R. L6 c5 N
2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells: Y9 E8 t1 J1 C3 {% W9 r! N$ a+ H
2005 Anchorage-independent growth of mouse male germline stem cells in vitro
( d6 q) R6 C" W  D2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor: n! }- g% V9 _+ B: ^; f2 r
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
( N% b: U' K: O8 O1 ]3 O2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment9 K6 _# ^% U8 ?- i
2005 Isolation of male germ-line stem cells; influence of GDNF/ R  F% r, t# D0 @+ X
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
% i% X' P9 {, o: h0 ~# b) L* y0 Y2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
5 M3 d2 K! X: [1 O2006 In vitro propagation of spermatogonial stem cells from KM mice7 l  n  r  E! Z: z
9 ?) `9 O5 ]# \" d. q
上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的