有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章/ Z. n& T, q, e3 a7 {0 w+ S' s
2002 Lactate inhibits germ cell apoptosis in the human testis
, L" S" H0 z) R4 u8 M4 ], D3 B2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture6 w$ Y. [& |# s' _7 g
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal& [7 a  i( I1 w9 \
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells- l! D( V. P( S/ C
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro5 p: r" W% N. r4 a1 [+ d7 o
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture8 `5 B3 o& l1 [1 }. d9 C
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis+ [/ J3 @, P# J# Y( s1 X+ D8 P' F" r
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
- \" z, [. [7 t' S) t" ^2 Z2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse
/ p) S/ a, c% w- y2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines8 R9 p) Z% v$ C
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
1 {/ W" `) l7 x; Q2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells% _( Y  S% K3 @$ ^- V
2005 Anchorage-independent growth of mouse male germline stem cells in vitro0 \# c/ u/ H5 P: ]2 V7 W! \7 q7 O" A
2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor
4 a% T( E4 t7 q9 h2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
' @" n, |1 F( t3 f& M' X+ ^" v2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
, c+ Z7 R/ h& [2005 Isolation of male germ-line stem cells; influence of GDNF( t4 {, o" d* @8 g+ x* I, d& D. T
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions# ]" [9 r3 c' \+ x( w2 W
2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
: [8 [% A2 w' h# c7 s" l! ^2006 In vitro propagation of spermatogonial stem cells from KM mice
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1 Q9 b/ F( y, u2 ?+ [* W! V6 V上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的