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本帖最后由 细胞海洋 于 2010-6-19 13:37 编辑 + P6 J3 v. B7 k) Z
8 B" l5 F2 n% d* U+ I" KFOREWORD
, n( u- P1 c$ mStem cell biology is emerging as a field in biology with tremendous therapeutic potential., T9 w2 }1 D# S
Making this potential a reality requires an international effort. The recognition0 ~- u0 ]) Z2 c
that such a promising yet multifaceted discipline needs fostering led to the establishment
% w/ K) V% x ]1 |of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the
) L( a/ d; Y a" c, c4 A9 VISSCR is the development of tools to ensure the success of stem cell researchers. What
7 }) `" z' l/ w- E& B* K/ N& xbetter way to do this than to collaborate with Current Protocols to develop this valuable3 L7 f5 @$ n7 B' [0 s
compendium of protocols in stem cell biology?
, a( r5 H; F! S, d: YStem cell researchers have developed a number of breakthrough techniques, from the
k0 N/ c6 o+ ^& L! oderivation and manipulation of pluripotent embryonic stem cells to purification and$ n0 _( }# R$ {& G( U0 g4 |( J
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories6 N. F5 S; `, T; z: U
have become leaders in the field as a result of developing such techniques. The more
7 V/ h) ^+ J2 O! ^efficient scientists are at implementing new and powerful methodologies in their own
4 X. `+ r% L4 Q$ |. ~: _; blaboratories, the faster stem cell biology will advance our understanding of normal, p! x; r8 g0 U
development and lead to the development of therapies. Thus, the availability of quality
7 w& K! N$ Z! Pprotocols will have amajor impact on the success of the entire field. Current Protocols has
0 {2 Q( v6 x* j+ X: D7 Ulong been the premier volume for proven in-depth protocols regarding many aspects of
% C0 ]- s w, A1 `5 _+ g) B3 N8 ]biology, and this volume on stem cell biology will prove a valuable addition to researchers% N; H1 i, _% F. O6 L
worldwide.
" w' t" y3 X; a# j4 pExperiments in stem cell biology must be interpreted with great caution as well as
@1 w& A1 M xopenness to alternative explanations. For example, the recently discovered phenomenon
$ Q" Y( n. ~: g2 B, j/ Oof cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral% ^4 Q7 C+ d) J5 {/ n# q. B1 Z/ M- l
tissues were initially misinterpreted as evidence for stem cell trans-differentiation.
3 W7 Z2 F, k2 q8 E, Q+ H3 ?" ~It is very important that this compendium of protocols highlight potential pitfalls as well) ^; _9 K* E3 q6 W0 n
as maintain the opportunity for clarification and correction when the need arises. The
) \, x9 m' M$ J# q) f& qfact that these protocols will be provided online will help ensure that researchers always2 ^2 u2 p, c- ~, v$ T% h
have the latest, most up-to-date protocols available to them.
" `$ w9 w' ~5 n2 S X* OThe stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine9 e8 h( g" ?" w
push to share information and interact so that the field can move forward quickly. There
1 b6 I( q5 l1 s- a) I& f* ais a drive to develop not only excellent basic research skills but to bring the findings/ U7 H1 R' k! f8 P7 O w6 k0 Z
to clinical use so that patients can benefit. As a Hematology Attending Physician at
5 i/ N6 T9 x X' ?Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia
& K# P# V* f( S# Y1 f, z$ R1 R" hand am drawn by the need to translate our research findings into therapies to help treat a/ L0 b: e4 g- F- }
number of diseases. The promise is great, but we need to deliver, and I believe Current* P) L: i- |4 L( _* b4 d4 R* A
Protocols in Stem Cell Biology will help tremendously.
U1 P, N2 d" \' R# wLeonard I. Zon
/ S P z$ ^. ?, q$ U0 E3 JCurrent Protocols in Stem Cell Biology
9 ~* c+ F N$ y* n1 jPublished online June 2007 in Wiley Interscience (www.interscience.wiley.com). s( N4 B3 P/ }* E0 L7 I5 y
DOI: 10.1002/9780470151808.scfores1: X/ ]5 A( D* I; l- O' Q
Copyright C 2007 John
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' b( ]: B# L& |- e& Y' Y4 ]附件为pdf全文,感谢原创提供者 ,大家回复之后就可以下载了
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