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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml.
4 x9 z1 d* ?' t! r1 X Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells
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# M- v: S; Z" @* ~, ?- yCaution：ECM gel may be stored up to 72 hours at 2-8 °C.8 J% V r, K9 m# A7 a
' x8 \4 F/ l& z0 i0 g+ X' a, H
Other Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.
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0 @/ D! A# P$ d ~* A' o. p' fProperties
5 o7 @% [7 B4 S3 w; Nsterility dialyzed against chloroform
! b% W) C K/ c9 cform liquid 3 w; `9 S$ D4 T' |9 H
concentration 8 - 12 mg/mL
0 i" j0 E! C5 d- Y- zsurface coverage 6‑10 μg/cm2
3 ?) T$ g3 ~3 ? c1 o5 Y6 k- Etotal impurities endotoxin, tested
! @+ }0 s' I0 Z; `- X6 Csuitability cell culture tested 4 u; i3 ~4 y2 C' A7 W, ]" Z
shipped in dry ice % S# R, I0 n! F4 |) x. V
storage temp. −20°C |
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总评分: 威望 + 5
包包 + 10
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发表于 2010-8-11 10:46
