PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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) ]5 c; q, S( i2 YCurrent Protocols in Stem Cell Biology 2007年完整版 1451页
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% E) a# c$ m5 I) Z& h1 F& bOnline ISBN:9780470151808
, _  v! m" ?' o- B' \DOI: 10.1002/9780470151808
1 ^* {5 I# ^" s3 i# q8 o: `Table of Contents
" d1 h1 k+ c0 w1. Foreword
2 d4 z, E3 ?9 X- S) v! u: b2. Preface  F2 p8 a$ C: Q" v- P" Q2 B* P
3. Chapter 1 Embryonic and Extraembryonic Stem Cells9 z) w- _# u& f6 i
1. Section A Isolation of Embryonic Stem Cells" S! w% L& c3 i$ v9 C/ E6 s
1. Introduction
4 t6 M0 X: h) E1 y2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
: @/ t! Q4 W. Y" _0 Z6 h; j' k1 \" A3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
3 ]) Z/ g9 ?8 R4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
1 `1 C. @+ X$ V& j0 l: [5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
( s% E' v$ s/ R3 r# R; x) [2. Section B Characterization of Embryonic Stem Cells% f5 M$ |7 W3 P
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
: I2 y2 E( R$ G5 f2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and' a: q1 z" ?5 L8 U3 f. x1 h. Y( u% L
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
7 {0 L* q: P" F8 {; h. V1 o3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
! W: @8 ~5 s& ^% I" n) v4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of% |; ?) F% M6 I
Pluripotency( i- h% j3 o9 e4 G# x+ E: k
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells7 F1 n4 A* f2 h# |  F* e
Using In Vivo Biotinylation
/ x; h, Q2 C2 ~( i+ @  r4 B/ b6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem
# a4 Q' [, }$ [+ k2 n/ mCells
2 e+ g- a3 K$ Z/ w7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
7 f$ `% `- o4 G2 v, l' JProfiling
1 \3 v# Q5 B3 z2 {  T7 X3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells3 Z5 E( |- ~2 F7 y
1. Introduction
* W. z" W+ _) _: [1 r2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
. \  Z" p% N6 w7 e; @- O3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
6 Q: P7 ~1 C* k' ~+ }2 U7 c4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
8 `; B& \9 ?' M% k9 o0 wMouse Embryonic Feeder Layer Cells, O1 h5 x- D+ C0 [2 I
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
8 ]( L0 @0 V) f$ I) L( g% [; j% `6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic( j, |0 ~: k. r  g
Stem Cells$ R- w# K+ c& l0 K" E: m* R" C1 q& a
7. Unit 1C.6 Isolation of Human Placental Fibroblasts( @$ |" V, g4 w: r
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic* Q1 x# p! v, O% h3 I% k- L
Stem Cells% m+ R2 ?) b0 h, X
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of, N1 ]9 W/ `- C4 j
ROCK Inhibitor
1 |8 p. l1 d; C$ f9 E2 t) S10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells7 a% t1 Z3 L; H" ~% r
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
- s3 P: ^; Z9 o6 S5 Z  r9 s. pStem Cells on Mouse Embryonic Fibroblast Feeder Cells
+ W9 E: N7 V( I, N* _& e12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
* p/ r8 f5 X, S6 _* B4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
& A0 P$ Y2 `; B  I! N- t3 _8 k1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
4 T5 W- T# g. N* s2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
+ A( B, ]3 ]4 D1 a+ JSuspension and Hanging Drop Cultures4 _. n7 \6 A, ~2 ]8 X* K6 K
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies7 C& a/ |% `5 Z6 g
and a Description of the Hematopoietic Blast Colony Forming Assay
7 P3 p& n7 A9 P4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically
  c- @  c0 N; ]! \( t1 |Defined Culture Conditions
. U, X! L5 t1 S) @& d4 h( D9 V+ m5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
% e% p% p4 I7 C: h2 \5. Section E Extraembryonic Lineages) L! y. U7 Q( z, p5 w' z; n: L& K
1. Introduction1 W9 o- N3 K2 h0 p+ f# P
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
6 }+ l5 Q- X2 L5 e; b7 w) b3 ?2 ginto Hepatocyte-Like Cells
0 M# K1 ?& J" ~& b0 R3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
% S# J7 j: }" A* f4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
6 j: z# p7 r3 Z5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells8 |; h6 p8 U; d6 n' _6 D
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
  u( r4 S: l- f2 O* O$ ^' m6 C7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use( G2 E9 c1 z8 }$ K- H8 J# A) Y! z
6. Section F Mesodermal Lineages
. o: F) T! u6 D% `1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
. g/ G( p6 f# G& |0 o  L' c2 o2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture, O$ w+ \% a- q( d1 Y' g8 I
with Endoderm in Serum-Free Medium! `& n' m; h+ N& j; A4 c4 \
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells- @, @4 s& ]: u& Y- y
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood& U4 P8 j9 n- L9 c/ n0 v1 S
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
8 J0 a2 Q! u- C0 s4 ~3 w, {6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation
* w+ V- ]" x+ ~with Stromal Layers5 ~; `$ e4 P! _" E7 C* \3 M0 |% b
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary0 Z+ q: J, P8 p0 _" O. A( m
Murine Hematopoietic Progenitors8 j- X' z$ U5 X0 `0 b$ s
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
7 a; `8 r' V) Q6 d  z" h( v  ]Human Embryonic Stem Cells
! a# H  R  o# N' q9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
" k% o0 w! t3 U3 Z# C0 [/ M+ h" e10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac. O; {7 u5 v: v& i" A4 S2 b) `
Progenitor Cells
7 X# C" g: ^$ |5 Y7 w- x5 U' h11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the$ ~2 U* U: w  S# r, B& k% m4 v! m
Hanging-Drop and Mass Culture Methods8 f; }" `, V+ O1 z; i$ Y
7. Section G Endodermal Lineages
3 t+ c* {# @; n3 @! D+ l+ O5 e1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
* I2 x& K. e2 x2 W/ }( e/ e# M2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
2 V8 p$ o8 J: T/ L5 x: ^3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm+ w7 Y/ H5 v6 w2 k- V
8. Section H Ectodermal Lineages' t' [) V/ W# P+ g# S! e% y$ T% s
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons5 R1 E( q9 m, S' u: n0 C% \4 u
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by- y& O7 e5 ~8 p; V; v
Two-Photon Microscopy9 \) W, R: W7 y6 Y' z& {
4. Chapter 2 Somatic Stem Cells
2 j) m! L7 P  h) {9 A1. Section A Hematopoietic Stem Cells- O( X, }0 {# @! v
1. Introduction" U" a5 r) b% J/ z
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density" d/ @8 J, t7 r6 b2 _% ?% N' y
Gradient7 G) Q. N3 Q; E, P2 C
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood$ _& W0 `9 x8 y$ `. I, y- o' n
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood0 M  Y6 G+ v" W8 _' E
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells$ ]$ e# K% @) u; Y, d4 I" Y
from Adult Mouse Bone Marrow
6 _9 n0 [2 f4 F, ]+ K" c0 ?6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche5 L, [; Y" o) I) j" ~: r
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
8 J  e& ]: R5 L0 _2 a; n# Z8 NEmbryonic Tissues- Y. H# p/ o' g- ~
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells5 R1 [; H3 n# Q9 H( W
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells# [( W$ g5 i# _- Y$ G! K; O- y: L
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta5 \# _, i& n; E; Q: ~4 x9 G
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
9 V* g  t* J0 Z$ }! U, N1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
: F1 @. `2 P! Z5 F/ _' z# ^Tissues
; h+ x9 j" W4 `4 u5 W2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
! ~" t; c- r0 Y4 N- Y3 ^6 z1 e3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
3 D1 ^: @  m: B" N/ Q$ X+ j3. Section C Cardiovascular Stem Cells. m) l6 N6 @& b. k# S* n: s
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood9 L2 e0 T1 u. d' t; d, G
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium+ k7 l/ y2 c: Q$ P% }
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
( s/ j9 s0 a1 R8 C4. Section D Neural Stem Cells3 l1 k9 q; l' h
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
5 h3 \" K( I& V1 b- C2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
/ \  N9 H6 n6 u. y* {/ D8 b# Z+ _# K3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
% v+ i0 \. X1 {4 E6 k, S! O  bCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
) `; ?0 C+ ~/ E& Q; OForebrain Neural Stem Cells8 H. d: X; C% U4 g6 B2 ?
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage" v+ K+ ^; \- Y& x5 l: L: u  H+ M
Progression, and Maturation
' G4 ^3 l: z+ h- C5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
2 w0 k9 M% [0 e* Q: c: {- Y  LNeurons from Rodent Brains2 ~' k; Q) ?4 y; x) R6 p0 d' }
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
1 ?; R6 z5 z9 C% l# H0 tTechnique
3 ^9 k1 _1 r" q6 D* ~4 O5. Section E Germline Stem Cells, ^9 z7 D! y' `+ d5 b
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
4 k& L( @: x2 B2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
8 I% R7 ]2 v( t" G! `9 i. n7 s  h6. Section F Gut Stem Cells* x! b5 _4 V) e
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
: G1 H3 R& ~6 Y. l8 F$ Y7. Section G Lung Stem Cells0 q# F5 r3 D, z& O
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells/ X; S. O+ x' E2 Q: k
5. Chapter 3 Cancer Stem Cells  d) I$ V* M2 r6 o1 m* }
1. Unit 3.1 Colon Cancer Stem Cells
7 J: v  C2 F7 e1 b, F$ P2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
& {+ A3 p$ [3 N, y: U" Z3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells  H6 c& _9 v  [) a1 }7 V$ \3 {% d
6. Chapter 4 Manipulation of Potency
+ q% a* y& P; c/ o0 d3 g7 A# L; c1. Section A iPS Cells
+ ^, j! ]1 d0 L: ], \7 d1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
1 H3 V$ Z1 x6 F4 F2 k. y# ~: A. K2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells. x  [5 @3 Z. x8 H  q% r2 n5 e
2. Section B Nuclear Transfer7 m' g/ K/ _  T- K0 h  {
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency4 c$ O% e) z4 D& i: t$ S4 c7 b
7. Chapter 5 Genetic Manipulation of Stem Cells
5 [: G3 F* P2 Y. i% k1. Section A Lineage Tracers in Stem Cells! j* v: D" _# @, r
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
3 H9 d8 `( j) p# R( U8 I2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing3 \5 I4 }4 m5 V. E
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
+ B$ p2 e& |  h" U4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium* m! B$ e8 r; Z
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
# d3 T" D' J. ^3 jNeocortical Neural Progenitors
, W  _; u( ^9 K: |/ k2. Section B Homologous Recombination in Stem Cells
& k6 b6 R& k1 c7 l7 Z. L5 F6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
8 N4 z- W' O% SHomologous Recombination; d7 ~3 T4 \3 @, c1 f, t0 Z
8. Appendix 1 Useful Information
# Y4 h; V. y" c8 c: K* L- f5 i" S1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research) P! p1 T, x5 j
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
" e/ b! B( T  ~# [( ?9. Appendix 2 Laboratory Equipment
( g; K) p1 k$ F3 }' Q7 U8 c! ]Standard Laboratory Equipment9 X" ]. S# J7 j3 t
10. Appendix Suppliers
3 |$ v* {$ B" Y3 OSelected Suppliers of Reagents and Equipment0 Y, K6 S9 K) M+ \3 l

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