PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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$ S# O. |: V2 k3 M  NCurrent Protocols in Stem Cell Biology 2007年完整版 1451页/ I9 D( b  T5 w! W/ J. r9 ]9 x0 Z0 I

' u5 h+ ?- ?) y  h5 e: C1 [& FOnline ISBN:9780470151808
# m3 I; W# D' m4 E  LDOI: 10.1002/97804701518087 @: w: Z. y! e3 _2 X% p, E5 g; s
Table of Contents' k! f! d; I( H3 K% N
1. Foreword
/ Z: [; I. N, E) ^2. Preface
0 h# R' D6 M8 s; @  X3. Chapter 1 Embryonic and Extraembryonic Stem Cells2 G1 z" G/ W7 S0 O6 Q
1. Section A Isolation of Embryonic Stem Cells6 `( [* S  @6 L! U% M8 F  E/ Q  o
1. Introduction1 _( t( y$ d! {5 b4 \
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells6 |" Z/ j7 g- V0 ~+ g3 B; Z# e9 i
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts' V# d6 ?* F# |6 W: v+ `
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
8 ]. }6 h: J& ~- L/ Y4 p  {5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
2 ^& |8 M* h% q' q* [' G8 D2. Section B Characterization of Embryonic Stem Cells
1 ]) m5 J  U5 ~3 [. y$ h1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells  _  M/ a6 d7 x$ c5 u
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
3 w0 a# |2 y, q# c1 l, N: w# w2 OEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform  o) P& j5 W" `, m6 M
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells  Q: T. s! f- I8 ?
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of; \. `5 b! i6 h  a5 E- |
Pluripotency; s: ?& k# b2 b
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells/ `( W! Q2 Q: {" d) f; n; e' _
Using In Vivo Biotinylation
9 _2 f  E! C) y3 V' }* X" x0 T6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem7 q0 Y2 o+ H, c3 `% Y  A, {
Cells
7 C+ N( }" Y9 g7 z; V; s8 r7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
6 Z: O1 [& _3 [! z. R6 I) m3 ]: TProfiling9 V6 @* N! l( H1 {
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
: L: H- _1 M! R: {+ d, X# M1. Introduction
, J- p6 V$ u1 g. s7 C& t2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
1 W: N% ^" R+ ^6 T" a+ w5 K3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
: E: i! o0 F, d! l/ Z6 d4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of* [" l. R" h, o; m
Mouse Embryonic Feeder Layer Cells9 i1 `3 s  e6 U7 L' S' T+ w0 v
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells0 a5 A: N5 K1 Q* P; i. o4 `+ ^
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic
& X, z! z4 A) \5 S7 _! ~Stem Cells8 t' n* t( i6 r* Q3 V+ c
7. Unit 1C.6 Isolation of Human Placental Fibroblasts
# q1 R) |0 k# I1 t' g9 {8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic2 f$ y+ r0 `% u& m, w) p' X/ d6 D' i
Stem Cells
7 g6 T) A9 z2 A* y9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of7 b( p% r) H5 c" ?/ v3 v' |
ROCK Inhibitor
; }, E0 j; J: g, Q2 r, h) \8 J10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
2 N$ y" T3 N+ V6 r# B7 H11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
5 O0 Q4 @+ e8 M6 W+ W6 [Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
) ?( s6 `$ {$ G/ n5 s* X# l12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers8 Y! _8 z8 b7 r7 v; i! Z  Q7 z
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells) C" T0 Q2 j7 l. o
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap* @9 U" K: r* x8 M# e+ L
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by" P- J& [& q2 k# @3 K
Suspension and Hanging Drop Cultures1 L% v: w$ m9 x7 @; j
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
- R& p$ |4 i; }8 P5 iand a Description of the Hematopoietic Blast Colony Forming Assay" T/ M! \/ c3 D% R# U+ ]
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically) R) J. B+ _$ I1 w& V$ B
Defined Culture Conditions
  @) e+ ~5 O- E% H4 F5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
) h( W4 S$ [7 b3 W3 h; O5. Section E Extraembryonic Lineages
# u: A7 I% H7 z. \1. Introduction
% M! R! `* x* X2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation. _& W3 w8 _' R* e+ W, q4 e
into Hepatocyte-Like Cells+ M9 d: {, ?9 J! _6 W9 c3 X9 j
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
; N3 z. Y7 T* Q/ F9 l" O4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
7 E7 \- @. q, r( r3 E0 |& v5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
, z* c8 O3 V& @4 z* k6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
) H. B" [8 @2 L* S) f# d! k7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use6 R" K4 _+ ~0 I# p$ o( }
6. Section F Mesodermal Lineages
( @2 ^) [- a* N0 R9 `$ P1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
5 S4 E; h$ J5 T3 x2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
4 c5 p% _  q0 O8 fwith Endoderm in Serum-Free Medium
9 s& ^& A' b! G/ D! |  K4 A) e3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
2 x% E" [3 g, y& H$ p, s4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
5 F( E" M/ G3 q  M* Y& V5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
) y! p9 W2 n% t& }) I6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation* }6 ?/ c% V% @  ~
with Stromal Layers
% t. ^- F. g. I8 c1 k+ r2 W. E7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary/ o6 |  B; v0 S- H8 E& `
Murine Hematopoietic Progenitors$ \2 N. D$ }1 I$ @/ Y" X& G
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from& U5 Z! L* @% n/ @$ L
Human Embryonic Stem Cells+ l$ E6 A( \3 w9 a7 M$ V. A
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells2 _: p" E. X5 N- d, E; n# }
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
6 ~, B1 @" e! W  M: v8 a9 q" @Progenitor Cells
6 l9 P( _- W2 H3 _+ I11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
0 `5 [, [, F+ w5 |# \6 w3 lHanging-Drop and Mass Culture Methods! F  G- Q+ }9 Q
7. Section G Endodermal Lineages# V& @2 W  Z- M
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells% F' |4 \& u$ k# F1 \& M
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
5 z* w5 H+ B; K$ E3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
0 A& I0 g0 v& `! `) V) u( T8. Section H Ectodermal Lineages% K$ E0 @! E8 w0 p
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
1 N, V; a9 a& J7 g2 ?2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by" t+ y4 j& k3 P5 A( Q
Two-Photon Microscopy: ^  m+ E  J' f. j+ L! x7 O
4. Chapter 2 Somatic Stem Cells
( G& o, C8 E  `6 z* Q* ^1. Section A Hematopoietic Stem Cells
6 F/ i' J  ^* H6 u+ N. S1. Introduction" R9 @( T" E3 F: S# j+ I% I
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density6 Z6 p( y3 r2 S5 P
Gradient3 L5 A. [8 u/ r! v2 I" c
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
# @9 [% j% z( I4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
9 c3 Z! a1 E7 L  G7 M* X$ g5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
" X7 r9 X1 M& m$ S" h9 Rfrom Adult Mouse Bone Marrow
1 ?% @" b. b2 x' @2 e0 V$ V: a% i3 h6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche! z2 [" e: W* Q) F
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine. X, ?; w$ }2 x0 E8 j8 t0 m3 M: r
Embryonic Tissues
& Y( a7 @$ F4 G. [( k' `8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells+ E" }1 Z7 y3 J6 x( `) ^* v7 b
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells: U) g8 v  d, v) v/ l( p
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
$ [1 i5 ~4 z0 e% B2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells5 q  ^" `2 Y: z. m  C
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
2 V) w* M! l+ S$ d" PTissues! I3 g; U9 C8 q/ M
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
3 {# ~3 d. _; A4 R9 |* F4 N% N3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells. P0 z6 i8 ~  U9 b% j
3. Section C Cardiovascular Stem Cells& n/ T3 }3 a% g+ E. q8 E, `; v
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood2 K% n! T# X& _& K3 u+ }
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium" |! b% l5 W7 a" M2 S6 c( e2 G* j& m, o
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells6 t  \9 n% @5 D% s3 ?
4. Section D Neural Stem Cells. F1 Z3 y! y% C) z! e1 a5 E
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains0 w0 }( t1 M* R( W' e
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
4 w7 A( Q8 `9 N0 ^9 c3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and5 Y# U% T" s# v3 H) U+ \1 d! d) X5 T
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
! @$ v2 P+ f6 {6 A+ O. e+ n. dForebrain Neural Stem Cells
. @. ]! R% j8 `5 K. W+ b+ p4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage
$ @; b- i9 z# f6 ~3 u  ~Progression, and Maturation
# r$ @  W9 X" U1 u4 z5 a' d: B5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic: j1 y+ u8 P5 J
Neurons from Rodent Brains! v0 B8 Z. Q% K1 A# q/ e( N# e
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere. ^# ~2 e6 b: Y% Y, `0 B
Technique
( D* f. V& ~0 _2 s) X5. Section E Germline Stem Cells
0 O& E" `$ D7 u& X- x6 ~) m1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila4 W: x. `, w, q5 `9 w* L
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis: Z: X# L5 M  Q% j9 K/ l0 i6 ^9 r
6. Section F Gut Stem Cells" a' R& T/ N2 v* V3 U
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
& |) \" y9 X* L9 J7. Section G Lung Stem Cells
7 Q! u" H, C# H, `1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
$ v) T& l! T4 l4 a5 G& Y! g5. Chapter 3 Cancer Stem Cells* N. h' b) V/ G3 p. Q
1. Unit 3.1 Colon Cancer Stem Cells
  d) {! V4 w% i" B2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model/ ^* k( c0 e( {
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
3 N0 C8 L: e: \; {. W0 D0 {+ j6. Chapter 4 Manipulation of Potency7 X* f8 S$ s9 E' p
1. Section A iPS Cells
- {- ~/ q* |1 O% z- e6 e* l1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
. X! u- F1 |" P2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells) |7 d" w+ @5 N3 M
2. Section B Nuclear Transfer
! a$ }# t4 m% V" V: {6 A9 j1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency' M# ~& N0 H* {- n" f- @
7. Chapter 5 Genetic Manipulation of Stem Cells
, `  a$ y( H$ o5 e6 Q1 L1. Section A Lineage Tracers in Stem Cells
: |, O6 c% x6 X* b) f$ W1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
( S& s8 C( `, i, e2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
! n( K& R; p; n9 P. ~2 E* ^. d3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells: E8 G) c9 ?# f* E( h/ P- e1 {) R
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium6 }+ y4 q+ m- ~# u1 ^
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian+ W3 H4 l3 v) i+ l  i3 j: U. Y* I) C
Neocortical Neural Progenitors7 q" t9 f( ^7 a3 u: O
2. Section B Homologous Recombination in Stem Cells# R. t" x1 ?( _% b7 w+ l
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
8 X  u7 h6 R  L  J8 u* o1 s2 dHomologous Recombination6 p( Q  i5 T$ U% T) R9 f
8. Appendix 1 Useful Information
% S% S! v- _' }1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
0 x6 T& i) }: ?9 R/ O2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
2 Q1 w- c7 [& G- _/ N, E0 H/ S7 |9. Appendix 2 Laboratory Equipment
) y8 E6 F% m4 y; D6 d. iStandard Laboratory Equipment
/ p$ p( I2 S8 r10. Appendix Suppliers( [+ v* H2 G$ Z. Y; O( S' d1 X4 T
Selected Suppliers of Reagents and Equipment9 o+ _1 m# P. h% q

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