PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页
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9 E% K- X9 }6 h, pOnline ISBN:97804701518089 ?" q# y$ z* j2 _1 q( G
DOI: 10.1002/9780470151808- M; a5 ^" B  `  V7 u: p* m
Table of Contents
3 Y: w9 Q" M. T* A1 ^1. Foreword
( J4 i- Y9 [' d2. Preface/ P: j  S" J' g# P# E
3. Chapter 1 Embryonic and Extraembryonic Stem Cells
& A# g4 t' K( M1. Section A Isolation of Embryonic Stem Cells7 i- O! t* _; v) [. Y; q, P5 H4 c
1. Introduction2 n  t+ ?7 R7 M/ I( _* p- b% i
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells7 v) k' X' w9 h$ `% @$ O" ]
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts' A& _' t% L; x( K: R, f3 I
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells1 h4 G3 f+ S% z8 `/ ]
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment: a7 }8 W+ P' g5 R8 S- S; W
2. Section B Characterization of Embryonic Stem Cells
* N  `' p( F& ~8 A& W" p  Y5 Q1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
# M5 U+ ?# }2 Y: ^3 l2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and; B1 b5 d* ], V8 i/ }; J
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
8 j1 o$ p/ h7 Z9 M3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
& j# ?+ U& X5 w8 N4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of' o0 ?) w! ^1 r  @
Pluripotency9 J3 c8 m8 k- W$ b
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells1 Z- ~" K9 T! B/ U) @& P8 \7 l
Using In Vivo Biotinylation3 }$ Q$ g8 x2 a, @9 \9 h% `5 d+ s
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem6 e. m* f# `- S& a
Cells, {) e* z2 g) d$ s' f% _5 X
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional! o1 u# F2 J8 B9 E
Profiling; R# D" Q9 g. X6 p. i# w
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells8 L/ G$ X7 a6 e+ A- f! b, v
1. Introduction! E9 l0 _; W" Q8 H; F4 r0 X7 v9 e
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro0 G6 x, A/ G% D: |9 @/ E( z. }
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
5 [' t' d" `4 Q" ^& Y" Y7 F8 s4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
6 P6 f/ a9 I7 q; ?- H& }Mouse Embryonic Feeder Layer Cells
: |; M! H; c$ w, q5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
/ `" c7 V' B5 i3 X& @7 R& n6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic$ x9 H4 S% C( X+ n
Stem Cells5 n, p* U# E2 X, P
7. Unit 1C.6 Isolation of Human Placental Fibroblasts5 H/ a# W% e" }6 \$ {& w
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
4 {1 T, ~$ l  b' m# Z+ V/ _Stem Cells7 d. h' t% Z8 I: C; n2 Z. R  U; b
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of2 @; u* v/ r$ G5 x
ROCK Inhibitor
% ~3 b6 Y( p) Y10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells' \2 j; R4 w) B
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
, Q  e- n0 Z1 X# z. o2 u# zStem Cells on Mouse Embryonic Fibroblast Feeder Cells
- b( v; ~; Z; q( N$ Q4 [" Z* N12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
5 r  ^* p4 L  Z3 S6 W9 k4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
; ?' i3 X' ?* z8 W, ?1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap0 {, X% h9 d0 J
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
1 G# H: f% u" o+ tSuspension and Hanging Drop Cultures
$ U' C3 \) d- `+ S3 c3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies. q- }3 b+ P8 K
and a Description of the Hematopoietic Blast Colony Forming Assay
) _  c' R, b8 T1 }) U4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically& g/ r+ V+ @  m+ D/ r
Defined Culture Conditions  a7 }+ _6 t# V6 N. H$ u$ N6 V
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells: ~: d4 t( h/ h5 P7 R
5. Section E Extraembryonic Lineages
6 _+ A0 p; S, i- U; v* H1. Introduction
- I. Y# G) a) X2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation$ ^" W$ E0 S# J$ m
into Hepatocyte-Like Cells2 X. x  p+ `6 E) ]: P" W( m
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta8 w2 W' F$ a7 d8 S6 l
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells5 a( }+ G) @2 S( L) N
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
. F; U. x3 q0 C2 J6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
9 [* ?# ~3 V" i, E7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use; x% O3 e6 T2 M' H! {* }) E) \6 |
6. Section F Mesodermal Lineages
+ \# M7 `) y) ^9 v" D- V: @4 T  t1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells% K' s2 s/ s- M7 ?
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
7 g3 r+ n- [% U  S' R$ c; ]. `with Endoderm in Serum-Free Medium- Y2 ?8 V: k7 o5 Q& H
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells' ^. c  Q; ^( f* T6 ?3 \/ r! X
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
+ L/ d5 C& Q; \1 U) ^4 f5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
, b  Z: G. w/ G8 r& N% G0 M6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation2 a6 X5 w" q6 u  a
with Stromal Layers
0 C5 q% L' U) \/ [. r7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary! u4 G7 k: Z9 F
Murine Hematopoietic Progenitors
! ~, L( a- r$ _( _& C! B8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from3 a4 A1 u: @! D. B9 p+ ~+ [
Human Embryonic Stem Cells& u. j) w" }4 ?5 h9 n$ B; X
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
" [4 F# J" Z/ x5 f10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac- C. {0 M+ L& d; U2 ~
Progenitor Cells1 g& q" c/ F& Z* V9 ?9 u
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
2 N1 k, Q* |9 ZHanging-Drop and Mass Culture Methods( @0 B! h  ^$ ~6 ^. X+ Y0 `
7. Section G Endodermal Lineages! p5 @  C; Z0 ~" N  T; m: \
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
( K+ a7 d4 e6 C2 Q' C1 A3 I7 t3 q, e2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells' Y( i  G! r2 x% ], i
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
# u- D, H  X9 Z, x8. Section H Ectodermal Lineages
% f" V/ y* H! S3 s4 H1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
# C0 E  |8 H7 D3 t4 c2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by6 i3 k! i- }4 G" M- R8 t1 Y2 V, w
Two-Photon Microscopy
% k) K3 F6 F5 S' \- L$ _% t) C4. Chapter 2 Somatic Stem Cells( `1 ^+ g7 D1 b* F0 c
1. Section A Hematopoietic Stem Cells
( B" y; S: H4 N; y3 V3 u1. Introduction
; @: _9 B+ k; n( U$ a9 s% I1 g2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
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3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood' j4 I4 S+ ?+ V4 q' G( V" Z7 Q
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
$ ]5 @5 K; O' I: F& Y5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
' w& K$ S4 u/ c( i8 w' bfrom Adult Mouse Bone Marrow& g7 C# U, v) N' i1 u3 T
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche, R% z. }* E, V; i5 K
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine" g" S- B/ A9 _6 i( U- D; C
Embryonic Tissues* @& ?9 u3 \2 U/ E# j! w
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
2 W( M* E; K2 b) }0 D% V; @9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
% a3 x2 Y" ], b: U: ^' y% I& ]10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
: G' b! S( W% T5 X  |2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
7 J/ B; T5 m2 w- S- G1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human8 u: s" \- H5 Q, H5 k2 [1 X
Tissues5 ~8 ~' s+ l5 _" Z
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
. v4 S  q: j- ^& L7 {" N3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
0 r6 G" ^$ U# i5 @3. Section C Cardiovascular Stem Cells% O+ e" U! y6 ?( ^/ Q9 H0 J
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood6 e* {' Z1 ?3 n, d8 W3 z. r
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium5 E) s( ~% G9 l5 A' Z6 U
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
/ u3 D: W& u; \7 p4. Section D Neural Stem Cells8 u! [$ i6 Y3 _
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains4 a, _" }( h& U  _& n/ R1 v
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells$ `0 L. w6 t3 G  b& q2 c
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
; [8 k. X1 V5 L" c) v# f" T1 tCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
* z+ P9 s) J& c& jForebrain Neural Stem Cells
8 T% B5 E$ ~  Y, t' c4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage5 x9 ]& z+ u# \& i2 Y, E
Progression, and Maturation1 [: V/ L5 V, `1 S) x4 u( k* L
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic. [% q0 q$ E8 B/ V4 h: m
Neurons from Rodent Brains
+ f. F  I8 V6 B2 c( X" h. i6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
# d: X! l/ V( M0 W+ ?" FTechnique$ W; g" z3 d: s
5. Section E Germline Stem Cells
1 u- K/ _. B3 U7 o8 _1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila# W  Y5 K& ^5 W0 w
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
3 J  n5 c4 C7 z( e6. Section F Gut Stem Cells% z2 R* O( M( V
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells% t3 T* _) _9 \) n
7. Section G Lung Stem Cells3 ~8 `$ h3 Z& p! g" f% Y
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells  c  t- M9 G& z$ x3 U  Z0 |7 F( w- T
5. Chapter 3 Cancer Stem Cells
8 ]' s4 c5 E% m0 s7 S4 D. [  |1. Unit 3.1 Colon Cancer Stem Cells7 i/ e* p0 X9 j  A% _
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model5 `8 n' J) L: d  V
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells2 _" M8 u$ a$ n+ v* T/ E$ i
6. Chapter 4 Manipulation of Potency
' K% R+ E. O( F/ f4 N* Q: B( U1. Section A iPS Cells
/ d7 e) H) n5 Q& O$ M1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
9 q1 ]% D6 A$ X/ s& I2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
# Q% ]! h( U: H! ^: Z; F/ w' o6 G2 m7 G2. Section B Nuclear Transfer
4 _2 q% F+ \2 E" b: l4 s/ Z( ?  z1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency& y) X& {8 M; o$ i& \
7. Chapter 5 Genetic Manipulation of Stem Cells
1 f0 T/ q) \) @$ D5 `7 y/ M1. Section A Lineage Tracers in Stem Cells" s. w) R$ ?* L% P5 a3 p
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
' M$ a! f" x( t: ^! v2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing1 c9 B+ J% G3 R4 I- R" p, U
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
# b4 L* B( e; R, R' _+ D4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
4 p" T4 F8 |) \6 X5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian7 S+ h9 A  c) s6 N* ^* m6 V; I
Neocortical Neural Progenitors
; M! n7 D# }( Z. W/ b) S& X7 k2. Section B Homologous Recombination in Stem Cells
# h5 ]: M: d# C) p6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by2 K. P- V* p  Q. G
Homologous Recombination
) W! Y4 X! c3 R. y8. Appendix 1 Useful Information, H+ V* w* m5 S' {1 F
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research( Y6 d6 u# n. D$ V
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
, Q( T& r. [) t% q% O% G5 G9. Appendix 2 Laboratory Equipment
2 v9 y5 b: |% c0 ]# DStandard Laboratory Equipment% i" [8 J& ?0 N" f/ O' W* J
10. Appendix Suppliers
, L. q3 [$ k" B1 JSelected Suppliers of Reagents and Equipment  k4 T, a! J* q; @
4 {3 v" B9 K) V! l& g, k! Z

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