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本帖最后由 qianqianlaile 于 2011-3-21 11:29 编辑 9 G: ?: T3 }! |& _- `6 ^
6 p' [( K1 |5 q/ kINTRODUCTION
) L, c' i I2 B c$ m! L& RCell therapy has tremendous potential in regenerative medicine, yet, there are concerns in 8 U g" _4 M; q7 i8 B: d5 r
the utility of cell therapy due to questions regarding different cell harvest and cultivation
6 ]# b @% j! j& \& {/ S6 Y. J+ vmethods (Haack-Sorensen et al., 2008; Mannello and Tonti, 2007). Bone marrow derived
( x5 Z5 ?% t2 U' F9 y1 Gstem cells have been identified for a number of years, and there are a number of
( O! O E* p9 K$ r2 Iongoing clinical trials exploring the safety and efficacy of their use for a number of
0 N! u# t1 R' kclinical applications (Battiwalla and Hematti, 2009; Sadan et al., 2009; Satija et al.,
. x% H8 b' r$ Z( S+ d' [; c/ B2009). There has been an increased interest in recent years in the potential of oral-# K* l& ^4 G: b/ S% u2 u3 x- N
derived stem cells for cell therapy, primarily because they can be derived from a readily . |* e9 S- I7 p. ~: H6 `
available source, extracted teeth (Gronthos et al., 2000; Miura et al., 2003; Seo et al.,
- F' V% @! V P; \( l0 s/ [2004). These cells exhibit multipotency and regenerative capacities characteristic of + r% q& D2 ?0 y1 `0 |& h
mesenchymal stem cells (Batouli et al., 2003; Shi et al., 2005) and have the capacity to
7 @( j- f0 b7 X: U9 ^* B: Wrepair and regenerate tooth structures in vivo (Krebsbach and Robey, 2002; Mao et al., 2006).
5 n$ r, c8 M7 A" b9 k Because oral-derived stem cells have been more recently identified, clinical
7 V! U3 C2 @' ]1 T9 [6 Cprotocols are still being developed for their use. Regardless of the specific protocol 3 X( c0 M/ Q$ n' ?0 ? K
used, most current cell therapy approaches rely upon ex vivo cell expansion in order to
2 U; ]! l* ^* b. l; uproduce sufficient cell numbers for transplantation. Though a wide variety of protocols
3 B5 S( i2 W# Y Nand culturing methods exist, one common aspect to most of them is the inclusion of
. n( a8 h2 o; y' Banimal sera for cell expansion, in that it contains a rich source of nutrients and growth
H6 B* K0 T! e: _/ v2 t* Mfactors (Mannello and Tonti, 2007). Despite the widespread standard use of animal / K6 q- j/ H2 T
sera for in vitro cell culture (Freshney, 2000), there are several problems which exist
' ~( P6 E% e z$ Z& u3 b+ Crelative to its use for clinical application.
/ O( d; `, u8 z2 L. H# E4 G2 u9 _One of the central issues regarding limitations in using animal sera for clinical cell ) ?, m1 P1 |) s7 B0 ~1 u. r
therapy protocols is that its components are highly variable and, in many cases,
m) l* M1 T) b9 s" B% \unknown. Though components of sera have identified, it has also been demonstrated
# R& Y; h& v+ b( Z4 hthat consistency between different lots cannot be assured (Price and Gregory, 1982). In
; C( R$ ^+ u A6 h2 Hthe context of multipotent stem cells, serum components and concentrations have
; P% `" `0 R3 |! o x" _significant impact on cell survival and proliferative capacity, phenotype, and multipotent
" g( B( W! ]/ I1 ppotential (Agata et al., 2009; Sotiropoulou et al., 2006). Additionally, for clinical use, the
# |5 K% j* Q4 F" Vinclusion of xenogeneic serum for cell expansion carries immunological risks associated
* {3 m; |6 H& {6 cwith the immunogenicity of serum proteins and the potential of transmission of prion
3 g& L* i& b: c9 ~: S* Kdiseases and zoonoses (Shahdadfar et al., 2005). These concerns have led to efforts
: e* \9 ^- _$ Naimed at incorporating FBS alternatives in cell expansion protocols, including the use of
1 Q, f& v0 g4 p8 D2 T% |) Uautologous and allogeneic sera, and the proprietary manufacturing of serum-free media 0 v4 x! o% O* _; F
formulations by different companies (Nakamura et al., 2008). Even with these approaches,
: B0 q: W" }3 F. C7 D there are limitations in the availability of both autologous and allogeneic
+ u4 ]- ` ?1 [' J( i/ hsera and companies do not freely disclose their proprietary “serum-free” media
" c( m6 ]4 f; [components. These factors not only prohibit clinical translation, but also limit
6 m E2 G/ B2 y! p2 V4 A: M3 Lwidespread use and study of more basic fundamental questions regarding specific
# n8 A/ V1 U: ?7 B# a! a C) amechanisms involved in the modulation of these media on cell function. As such, there
# D1 }/ L% Q5 @1 aexists a need for the development of chemically-defined media which can propagate the6 z/ v. Z" h% Y! \
cultivation of stem cells without adversely affecting cell function and phenotype ; V& D6 I) S$ ]& c- v
(Mannello and Tonti, 2007).
. R+ i C0 ~3 N0 J d" p( x- ^In this study, we aimed to develop a serum-free media (K-M) for the expansion o
& k, T) v6 A) ^dental-derived stem cells, including stem cells-derived from exfoliated deciduous (baby)
5 `$ T/ J( c- ?3 Ateeth (SHEDs) and periodontal ligament stems cells (PDLSCs). Cell expansion in this ( d8 ^& v( I! A0 @3 @6 n
media was compared to standard FBS containing media used to culture these cells, 7 i2 T3 _% f g
as well as three other serum-free media formulations (two of which are commercially available) # n% |% q* d+ n% l9 R, x; P
used for culture of mesenchymal stem cells. Additionally, through 9 j! w8 F' r/ i) x
differentiation assays and microarray analyses, multipotency and differential gene 6 @: }. H8 o2 }, b, j
expression of 84 stem cell associated genes was examined between cells cultured in K-( N4 u6 G( i) X2 Z/ X
M vs. those cultured in FBS- containing media. |
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发表于 2011-3-20 08:21
8 l7 ~) n+ H5 i Q; H' G
，脑子里也没有独家生产这个概念
，所以没理解。6 J; r! s! V6 Z" Q