|
 
- 积分
- 429
- 威望
- 429
- 包包
- 1768
|
本帖最后由 qianqianlaile 于 2011-3-21 11:29 编辑 # S/ {' u$ x* M* W) [" V. @
, V! M5 |' m( O& R, DINTRODUCTION4 b" T! Y- N3 f0 R0 J
Cell therapy has tremendous potential in regenerative medicine, yet, there are concerns in " N- A. g% [" F0 e5 e9 \
the utility of cell therapy due to questions regarding different cell harvest and cultivation
# V; [) D3 D8 F) w: Smethods (Haack-Sorensen et al., 2008; Mannello and Tonti, 2007). Bone marrow derived
9 D/ _4 x* F# P+ U) ?" ^) ^- Gstem cells have been identified for a number of years, and there are a number of
1 j2 L9 b; u/ X pongoing clinical trials exploring the safety and efficacy of their use for a number of
4 O3 S! U. p M& \0 }7 mclinical applications (Battiwalla and Hematti, 2009; Sadan et al., 2009; Satija et al., . }4 J4 g1 _, o# W- X, n, F
2009). There has been an increased interest in recent years in the potential of oral-
3 Q+ D9 Q" z8 q' Mderived stem cells for cell therapy, primarily because they can be derived from a readily
1 b/ Z+ e$ s2 f( \ gavailable source, extracted teeth (Gronthos et al., 2000; Miura et al., 2003; Seo et al.,
( S2 ?/ D* T" d2 f2004). These cells exhibit multipotency and regenerative capacities characteristic of
$ j* M" ^" _) ?# M: s* K" Umesenchymal stem cells (Batouli et al., 2003; Shi et al., 2005) and have the capacity to ( L1 `. b: k$ W* R) f$ o/ V; E
repair and regenerate tooth structures in vivo (Krebsbach and Robey, 2002; Mao et al., 2006).
3 T1 U9 A- f& [$ _ Because oral-derived stem cells have been more recently identified, clinical
1 e9 g9 h- e; L) x8 qprotocols are still being developed for their use. Regardless of the specific protocol 4 L! @& H4 r V2 v8 E! l1 N- e4 i4 e9 K
used, most current cell therapy approaches rely upon ex vivo cell expansion in order to , n9 `5 a! F, w5 v5 ?
produce sufficient cell numbers for transplantation. Though a wide variety of protocols
' p* G2 A" s2 V9 A9 S6 U& B8 A6 Vand culturing methods exist, one common aspect to most of them is the inclusion of
+ ^ m( y# {, x! t3 V! nanimal sera for cell expansion, in that it contains a rich source of nutrients and growth ! {0 V5 w1 r+ m# F. P
factors (Mannello and Tonti, 2007). Despite the widespread standard use of animal
" H6 t: d5 [* _/ E: D% @sera for in vitro cell culture (Freshney, 2000), there are several problems which exist # F, B! o1 e5 |2 C! i2 s+ b
relative to its use for clinical application.
, b% ]0 J- V% @9 oOne of the central issues regarding limitations in using animal sera for clinical cell : I9 E% l( V( C5 O+ ^, z a9 X- x
therapy protocols is that its components are highly variable and, in many cases, & y2 [* e; Z, c/ Y
unknown. Though components of sera have identified, it has also been demonstrated
/ c1 U! \ N2 ?' ^* `* Wthat consistency between different lots cannot be assured (Price and Gregory, 1982). In 4 n2 p9 ?* ~& ^4 y( A0 Y
the context of multipotent stem cells, serum components and concentrations have 5 L! L# b: X6 h% ]0 |3 |" E
significant impact on cell survival and proliferative capacity, phenotype, and multipotent - n0 l# A: u& m, c
potential (Agata et al., 2009; Sotiropoulou et al., 2006). Additionally, for clinical use, the
, ]9 Y6 `" z8 e& Z4 Ainclusion of xenogeneic serum for cell expansion carries immunological risks associated
- n8 e) h' w. U+ f* V l- Awith the immunogenicity of serum proteins and the potential of transmission of prion
5 j0 U+ Z; K9 h& q4 I8 z2 ^diseases and zoonoses (Shahdadfar et al., 2005). These concerns have led to efforts % J4 [$ T) N: S6 ]* M& d
aimed at incorporating FBS alternatives in cell expansion protocols, including the use of
& K; V8 ] k u6 u- q3 Vautologous and allogeneic sera, and the proprietary manufacturing of serum-free media
, w/ j6 l0 e% O* @formulations by different companies (Nakamura et al., 2008). Even with these approaches,
! V9 a+ h0 Y* V- n/ \2 q. @( [ there are limitations in the availability of both autologous and allogeneic : R$ T' C' a& Y4 H
sera and companies do not freely disclose their proprietary “serum-free” media / Z8 t+ z* z& `! o T: d$ O+ o
components. These factors not only prohibit clinical translation, but also limit
7 T2 l* G2 Y( b9 k) C7 Bwidespread use and study of more basic fundamental questions regarding specific $ g) z; b7 Z0 y' s
mechanisms involved in the modulation of these media on cell function. As such, there 5 v Y+ k. T; F6 N
exists a need for the development of chemically-defined media which can propagate the2 u {- T/ X G+ ]1 f1 j/ z$ L
cultivation of stem cells without adversely affecting cell function and phenotype . g8 s8 Q6 ^3 [9 s1 q7 L3 u
(Mannello and Tonti, 2007).
9 K( i; ~: D5 q3 zIn this study, we aimed to develop a serum-free media (K-M) for the expansion o2 o& d s* s% Y( b
dental-derived stem cells, including stem cells-derived from exfoliated deciduous (baby)
3 [( A8 [3 N) X6 a( Xteeth (SHEDs) and periodontal ligament stems cells (PDLSCs). Cell expansion in this
3 y# z2 x# ?' P- |* y* L0 f% xmedia was compared to standard FBS containing media used to culture these cells,
8 o/ D5 q* I2 | r/ Nas well as three other serum-free media formulations (two of which are commercially available) 0 n& L/ k5 F/ [) E
used for culture of mesenchymal stem cells. Additionally, through
% j% G2 }$ ~$ Z/ p; c u9 jdifferentiation assays and microarray analyses, multipotency and differential gene
" a% f8 g: g4 y1 O' Aexpression of 84 stem cell associated genes was examined between cells cultured in K-
1 [6 A5 s: T& OM vs. those cultured in FBS- containing media. |
|
发表于 2011-3-20 08:21
，脑子里也没有独家生产这个概念
，所以没理解。