有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章
% }: O" M& a- p4 g2 B5 |: V2 Z, b8 {2002 Lactate inhibits germ cell apoptosis in the human testis" ]" C  v, A9 ?. O
2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture
1 r& Z0 L. m5 p+ o/ f, C/ u2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal8 o1 q4 y$ U) |
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells+ R+ I8 H: Q, \0 c
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro& H; A1 ~7 o# d4 [
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture
& o% j9 y7 ~+ K2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis2 X- Z# D; f% f/ f, Y# ?0 j
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
$ ^1 l$ k0 ^1 _! E% [7 G2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse
8 y: j9 h1 M- S( V% `2 d2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines& R4 V4 r3 Y2 Q0 m9 j! p+ a
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
7 l* C3 n' O5 F; Z* S0 ?2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells
5 u" `- n$ Z' P4 |4 Z2005 Anchorage-independent growth of mouse male germline stem cells in vitro
" Q! M4 _; G$ |; N/ O" {" y2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor$ K% }  c1 T6 _0 y, ^+ N/ w9 w
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
0 u; D- v3 g3 q4 B/ N  [) N# J  U1 b2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
& c$ i) I5 H: e! y8 z7 W* m2 w3 a2005 Isolation of male germ-line stem cells; influence of GDNF) J" }& f, w+ h  O. J
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
9 t0 ?. ~9 h/ B9 y/ U" ^2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture8 i% I6 `% ~$ {5 [
2006 In vitro propagation of spermatogonial stem cells from KM mice# r( `- O  L4 z+ p' d2 Z  o* W
$ `7 p: b, G  {2 O% |& }) [
上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的