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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml.
* d4 l6 H# `/ ~3 N! P# b# I; } Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells 7 l. J$ a: e8 w$ x: ~+ U% a* ]4 z
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Caution：ECM gel may be stored up to 72 hours at 2-8 °C. r2 G+ m$ [8 t5 I
! o0 A" j' t! m$ e8 f* AOther Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.* c- R2 l, u, I( m" V
9 M6 |7 i# J7 c4 l, x$ M
Properties8 M) U1 Z6 t4 v$ b% b! O
sterility dialyzed against chloroform ! @2 q% V: G5 J2 o3 C7 \9 ], U+ O8 v
form liquid
/ T; k* ]' p' zconcentration 8 - 12 mg/mL 5 l7 Z) F) l7 q4 I% P5 Y- g3 }
surface coverage 6‑10 μg/cm2 / z8 F2 l; x# |
total impurities endotoxin, tested
0 k/ Z: ?$ Y" b2 b/ U5 B6 \4 x. w Nsuitability cell culture tested / ^ U2 w8 T, c5 E
shipped in dry ice
/ a" z) _) l1 nstorage temp. −20°C |
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总评分: 威望 + 5
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发表于 2010-8-11 10:46
