有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章
9 }* [( V7 _% M% {1 ?2002 Lactate inhibits germ cell apoptosis in the human testis$ Q* I2 n' _2 q$ @
2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture
& A) K' n# l* l/ j2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal
6 `$ W1 u/ j, d( H+ ]& F2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1 `$ b9 f% H; L, h1 u& y
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro
7 |: P5 p0 R  n1 L# Y3 N2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture
8 O. s" E2 X2 k, Z/ D: `2 J2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis
- U9 N& h/ G6 O: M2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
: ?4 M7 i) `9 L- `. i  ?2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse# k, X. u5 m; f* c9 e6 _
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines2 m/ B7 @0 V1 k$ _! t2 [2 R
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
, \5 a4 Z6 p7 E. c7 B# Z2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells  F* j+ s3 H1 e$ D$ y# Y% a
2005 Anchorage-independent growth of mouse male germline stem cells in vitro. c# I; g5 I$ G
2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor- I' V- g, h2 R* p3 Y! _1 ^8 v
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat' |8 \' X0 j$ }4 f% B
2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
9 R, a- y( r) F' E! o2005 Isolation of male germ-line stem cells; influence of GDNF$ t4 N/ |7 O! }0 b' F4 \
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions) ?3 }) w* G1 s( U4 z* g  G/ X
2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture+ O: T5 V' g7 O3 _
2006 In vitro propagation of spermatogonial stem cells from KM mice
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上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的