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- 积分
- 292
- 威望
- 292
- 包包
- 697
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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml. 6 E8 }( W! }4 H
Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells . c4 p' p& ~# g* E/ l8 {
+ k2 k2 u/ k w: a( }
Caution：ECM gel may be stored up to 72 hours at 2-8 °C.
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( z+ y# X; `, t% u2 nOther Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.+ v3 m, q2 H3 |& k( u9 \3 Z
3 C0 |" ]2 R3 ]( c* M0 w- {Properties
' J& `9 x* q$ R5 H! Q$ ~7 msterility dialyzed against chloroform
8 K5 j5 F, u- [* Y3 |8 k; Bform liquid & g9 J8 ?: M3 n- ^0 a
concentration 8 - 12 mg/mL
! I& R) b' m' ?surface coverage 6‑10 μg/cm2
( v: h5 a! O6 A: X6 _1 Qtotal impurities endotoxin, tested
) \7 l2 [) j) z- c/ f& f$ tsuitability cell culture tested
" t# X2 Z% v6 L9 ashipped in dry ice 4 A8 H% ?! m" l9 b7 S4 U
storage temp. −20°C |
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总评分: 威望 + 5
包包 + 10
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发表于 2010-8-11 10:46
